Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 183589..184316 Replicon plasmid pLH94-1
Accession NZ_CP035203
Organism Klebsiella pneumoniae strain LH94

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag EQH49_RS26895 Protein ID WP_110222749.1
Coordinates 183589..183900 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag EQH49_RS26900 Protein ID WP_011251286.1
Coordinates 183897..184316 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQH49_RS26855 178791..178856 - 66 WP_171005074.1 type II toxin-antitoxin system RelE/ParE family toxin -
EQH49_RS26865 179004..180007 + 1004 Protein_159 IS3 family transposase -
EQH49_RS26870 180027..180995 + 969 WP_194163332.1 IS5 family transposase -
EQH49_RS26875 181053..182102 - 1050 Protein_161 IS66 family transposase -
EQH49_RS26880 182151..182498 - 348 WP_004114612.1 IS66 family insertion sequence element accessory protein TnpB -
EQH49_RS26885 182495..182833 - 339 WP_023288266.1 IS66 family insertion sequence hypothetical protein -
EQH49_RS26890 182926..183384 + 459 Protein_164 DDE-type integrase/transposase/recombinase -
EQH49_RS26895 183589..183900 + 312 WP_110222749.1 type II toxin-antitoxin system HigB family toxin Toxin
EQH49_RS26900 183897..184316 + 420 WP_011251286.1 helix-turn-helix domain-containing protein Antitoxin
EQH49_RS26910 184463..185431 + 969 WP_074422534.1 IS5 family transposase -
EQH49_RS26920 185743..185979 + 237 Protein_168 IS66 family insertion sequence element accessory protein TnpB -
EQH49_RS26925 186035..186460 - 426 Protein_169 IS5/IS1182 family transposase -
EQH49_RS26930 186492..186827 - 336 Protein_170 conjugal transfer protein TraI -
EQH49_RS26935 186841..187629 - 789 WP_040217257.1 hypothetical protein -
EQH49_RS26940 187650..188093 - 444 WP_048298738.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..202829 202829
- inside IScluster/Tn - - 178977..202384 23407


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12330.08 Da        Isoelectric Point: 9.7248

>T117368 WP_110222749.1 NZ_CP035203:183589-183900 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSPDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Download         Length: 312 bp

>T117368 NZ_CP035203:183589-183900 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCCGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTGAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15377.46 Da        Isoelectric Point: 4.4420

>AT117368 WP_011251286.1 NZ_CP035203:183897-184316 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT117368 NZ_CP035203:183897-184316 [Klebsiella pneumoniae]
ATGATTACCGACACTGCAAAAGCCATTGAAGCAACCAAACAACTCGTAGCTGCCGTCCCCTTCCTAGGGGGCAGTTCTTC
CGAGAGCGATTACCGCGAAGCGATGGAGCTGGTGGATTATCTGATCGAGAACGACGACGAGAACCCACTTATTGACTTCC
TGGCGAGTAAGATTGCCGACTATGAAGATAACAGCCCTCGTTTCGCTGAGTTCAACAAAGCTATTGCTGAAATACCAGTC
GGCGTTGCACTTCTGCGAACCCTGATTGACCAGCACAAGCTGTCTTATTCAGACCTTAAGGATGAGATCGGATCTAAATC
ATTGGTCAGCCAAATCCTGTCAGGGCAGCGCTCTTTGACCATCACTCACATAAAAGCTCTTTCCGCGCGCTTCGGTGTAA
AACCTGAGTGGTTCCTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References