Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 2498..3128 Replicon plasmid pLH375-4
Accession NZ_CP035199
Organism Klebsiella pneumoniae strain LH375

Toxin (Protein)


Gene name higB Uniprot ID A0A3V9PY09
Locus tag EP126_RS26025 Protein ID WP_001708474.1
Coordinates 2793..3128 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A704FLZ1
Locus tag EP126_RS26020 Protein ID WP_001708475.1
Coordinates 2498..2788 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EP126_RS26015 792..2465 + 1674 WP_152660257.1 hypothetical protein -
EP126_RS26020 2498..2788 - 291 WP_001708475.1 type II toxin-antitoxin system MqsA family antitoxin Antitoxin
EP126_RS26025 2793..3128 - 336 WP_001708474.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EP126_RS26030 3333..3659 - 327 WP_060647093.1 hypothetical protein -
EP126_RS26035 3855..4889 - 1035 WP_152660219.1 hypothetical protein -
EP126_RS26040 5196..5498 - 303 WP_152660220.1 hypothetical protein -
EP126_RS26045 5570..5887 - 318 WP_152660221.1 hypothetical protein -
EP126_RS26050 6048..6341 - 294 WP_064163802.1 hypothetical protein -
EP126_RS26055 6478..6999 - 522 WP_152660222.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
EP126_RS26060 7009..7335 - 327 WP_152660223.1 hypothetical protein -
EP126_RS26065 7338..7817 - 480 WP_152660224.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..43433 43433


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(45-104)

Antitoxin

Antitoxin MqsA/HigA-2

(21-94)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12615.30 Da        Isoelectric Point: 5.2407

>T117347 WP_001708474.1 NZ_CP035199:c3128-2793 [Klebsiella pneumoniae]
MEYYEFVETSVFTREMKTLLSDDEYKEFQTFLIENPEAGDLIVGTGGCRKVRWSRQGTGKSSGVRAIYYFYNPAGRLYML
IIYPKSEKDSLTAAEKNQLKAVVAGFKGEEG

Download         Length: 336 bp

>T117347 NZ_CP035199:c3128-2793 [Klebsiella pneumoniae]
ATGGAATACTACGAGTTTGTAGAAACCTCTGTGTTCACGCGAGAGATGAAAACTCTACTCAGTGATGATGAATACAAAGA
GTTTCAAACGTTCCTCATCGAGAACCCTGAAGCAGGGGATTTGATTGTTGGAACTGGCGGTTGTCGCAAGGTTAGATGGT
CAAGGCAGGGAACAGGTAAAAGTTCAGGTGTCCGGGCTATTTACTACTTCTACAATCCGGCAGGCCGCTTATACATGCTT
ATCATCTATCCAAAGAGTGAGAAGGATAGTTTGACAGCAGCAGAGAAAAACCAGCTCAAAGCGGTTGTAGCGGGTTTTAA
AGGAGAAGAGGGCTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10909.62 Da        Isoelectric Point: 10.0073

>AT117347 WP_001708475.1 NZ_CP035199:c2788-2498 [Klebsiella pneumoniae]
MRKELFDDLIASAKEAVEIHQGLREPARVTRFERPDVKAIRAKVHMKQDDFASLIGVSSSLVQSWEQGLRTPAGAALKLL
RVIERKPEFVQELREA

Download         Length: 291 bp

>AT117347 NZ_CP035199:c2788-2498 [Klebsiella pneumoniae]
ATGCGTAAAGAGCTTTTTGATGATCTGATTGCTTCTGCTAAGGAAGCTGTAGAAATCCATCAGGGTTTACGTGAGCCAGC
TCGCGTGACCCGTTTTGAACGTCCAGATGTAAAAGCTATCCGGGCTAAGGTTCATATGAAACAAGATGATTTTGCCAGCC
TGATCGGGGTTAGCTCTTCTCTTGTTCAGTCATGGGAACAGGGGTTAAGAACTCCGGCAGGTGCAGCGTTAAAGCTGTTG
CGTGTCATCGAGCGTAAACCTGAGTTTGTTCAGGAACTACGCGAAGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3V9PY09

Antitoxin

Source ID Structure
AlphaFold DB A0A704FLZ1

References