Detailed information of TA system TA117278

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): bsrG-as-bsrG/-
• Location: 2232187..2232351
• Replicon: chromosome
• Accession: NZ_CP035165
• Organism: Bacillus subtilis strain SRCM103881

Toxin (Protein)

• Gene name: bsrG
• Uniprot ID: L8EAY0
• Locus tag: EQI56_RS11465
• Protein ID: WP_009967548.1
• Coordinates: 2232187..2232303 (+)
• Length: 39 a.a.

Antitoxin (RNA)

Gene name	as-bsrG
Locus tag	-
Coordinates	2232298..2232351 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQI56_RS11425	2227324..2227716	+	393	WP_019712881.1	hypothetical protein	-
EQI56_RS11430	2227737..2227988	+	252	WP_019712880.1	phage holin	-
EQI56_RS11435	2228116..2229252	+	1137	WP_019712879.1	tetratricopeptide repeat protein	-
EQI56_RS11440	2229242..2229418	+	177	WP_019712878.1	hypothetical protein	-
EQI56_RS11445	2229415..2230689	-	1275	WP_128472254.1	Y-family DNA polymerase	-
EQI56_RS11450	2230682..2231014	-	333	WP_109962767.1	YolD-like family protein	-
EQI56_RS11455	2231188..2231523	+	336	WP_041054825.1	hypothetical protein	-
EQI56_RS11460	2231567..2231926	-	360	WP_041338710.1	hypothetical protein	-
EQI56_RS11465	2232187..2232303	+	117	WP_009967548.1	type I toxin-antitoxin system toxin BsrG	Toxin
-	2232298..2232351	-	54	NuclAT_1	-	Antitoxin
-	2232298..2232351	-	54	NuclAT_1	-	Antitoxin
-	2232298..2232351	-	54	NuclAT_1	-	Antitoxin
-	2232298..2232351	-	54	NuclAT_1	-	Antitoxin
EQI56_RS11470	2232395..2232853	-	459	WP_109962768.1	SMI1/KNR4 family protein	-
EQI56_RS11475	2232866..2234752	-	1887	WP_109962769.1	HNH endonuclease	-
EQI56_RS11480	2234885..2235418	-	534	WP_109962770.1	SMI1/KNR4 family protein	-
EQI56_RS11485	2235503..2235753	-	251	Protein_2189	hypothetical protein	-
EQI56_RS11490	2235933..2236817	+	885	WP_109962771.1	endonuclease YokF	-
EQI56_RS11495	2236860..2237333	-	474	WP_109962772.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2116141..2264885	148744

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (1-38)

Sequences

Toxin

Protein

• Length: 39 a.a.
• Molecular weight: 4336.39 Da
• Isoelectric Point: 10.1022

>T117278 WP_009967548.1 NZ_CP035165:2232187-2232303 [Bacillus subtilis]
MTVYESLMIMINFGGLILNTVLLIFNIMMIVTSSQKKK

Nucleotide

• Length: 117 bp

>T117278 NZ_CP035165:2232187-2232303 [Bacillus subtilis]
ATGACTGTTTACGAATCATTAATGATAATGATCAATTTTGGCGGATTGATATTAAATACCGTCTTGTTGATCTTCAATAT
AATGATGATTGTAACGTCAAGCCAAAAGAAAAAATAG

Antitoxin

Nucleotide

• Length: 54 bp

>AT117278 NZ_CP035165:c2232351-2232298 [Bacillus subtilis]
AAATCTGCGTAGGCCTAACCCTTCAGGTGTCCAAACTCAAGGGAAGGTCTATTT

Structures

Toxin

• AlphaFold DB: A0A7Z7EVU9

Antitoxin