Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 165880..166415 Replicon plasmid unnamed1
Accession NZ_CP035137
Organism Enterococcus faecium strain SRCM103341

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag EQI29_RS14140 Protein ID WP_002314366.1
Coordinates 165880..166152 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag EQI29_RS14145 Protein ID WP_002297422.1
Coordinates 166152..166415 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQI29_RS14110 161315..162487 - 1173 WP_047928096.1 S8 family serine peptidase -
EQI29_RS14115 162517..163245 - 729 WP_002295679.1 peptidase -
EQI29_RS14120 163505..164053 - 549 WP_002292681.1 hypothetical protein -
EQI29_RS14125 164054..164911 - 858 WP_002292680.1 AAA family ATPase -
EQI29_RS14130 165197..165484 - 288 WP_002292679.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family -
EQI29_RS14135 165474..165803 - 330 WP_002292678.1 AbrB family transcriptional regulator -
EQI29_RS14140 165880..166152 - 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
EQI29_RS14145 166152..166415 - 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EQI29_RS14150 166649..167557 + 909 Protein_186 IS982-like element ISEfm1 family transposase -
EQI29_RS14155 167560..168735 - 1176 WP_010728556.1 MFS transporter -
EQI29_RS14160 168738..170987 - 2250 WP_002342422.1 alpha-galactosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid ClpL - 1..211011 211011
- flank IS/Tn - - 160807..161391 584


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T117253 WP_002314366.1 NZ_CP035137:c166152-165880 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T117253 NZ_CP035137:c166152-165880 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT117253 WP_002297422.1 NZ_CP035137:c166415-166152 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT117253 NZ_CP035137:c166415-166152 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References