Detailed information of TA system TA117230

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 142211..142465
• Replicon: plasmid pEC25-1
• Accession: NZ_CP035124
• Organism: Escherichia coli strain EC25

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: EQK40_RS24400
• Protein ID: WP_015387399.1
• Coordinates: 142211..142417 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	142404..142465 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQK40_RS24365	137458..138369	-	912	WP_000440183.1	carbamate kinase	-
EQK40_RS24370	138380..139600	-	1221	WP_000410951.1	arginine deiminase	-
EQK40_RS24375	140493..140975	+	483	Protein_173	VENN motif pre-toxin domain-containing protein	-
EQK40_RS24380	140921..141367	-	447	Protein_174	incFII family plasmid replication initiator RepA	-
EQK40_RS24385	141360..141434	-	75	WP_032336874.1	RepA leader peptide Tap	-
EQK40_RS24395	141670..141927	-	258	WP_000083833.1	replication regulatory protein RepA	-
EQK40_RS24400	142211..142417	-	207	WP_015387399.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	142404..142465	+	62	NuclAT_0	-	Antitoxin
-	142404..142465	+	62	NuclAT_0	-	Antitoxin
-	142404..142465	+	62	NuclAT_0	-	Antitoxin
-	142404..142465	+	62	NuclAT_0	-	Antitoxin
EQK40_RS24410	142604..142786	-	183	WP_000968309.1	hypothetical protein	-
EQK40_RS24415	142887..143503	+	617	Protein_179	IS1 family transposase	-
EQK40_RS24420	143541..145112	-	1572	WP_023149734.1	IS66 family transposase	-
EQK40_RS24425	145132..145479	-	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
EQK40_RS24430	145479..146156	-	678	WP_001339397.1	IS66 family insertion sequence hypothetical protein	-
EQK40_RS25720	146211..146300	+	90	Protein_183	IS1 family transposase	-
EQK40_RS24445	146601..146813	-	213	WP_005012601.1	hypothetical protein	-
EQK40_RS24450	146947..147273	-	327	Protein_185	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / aac(3)-IId / mph(A) / sul1 / qnrB91 / qacE / aadA16 / dfrA27 / ARR-3 / aac(6')-Ib-cr / tet(A) / sitABCD / aadA5 / blaOXA-1 / blaTEM-1B / blaCTX-M-3 / qnrS1	iutA / iucD / iucC / iucB / iucA	1..227349	227349

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7720.23 Da
• Isoelectric Point: 9.3127

>T117230 WP_015387399.1 NZ_CP035124:c142417-142211 [Escherichia coli]
MKYLNTTDCSLFLAGRSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T117230 NZ_CP035124:c142417-142211 [Escherichia coli]
ATGAAGTACCTTAACACTACTGATTGTAGCCTCTTCCTTGCAGGGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGGG
GAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT117230 NZ_CP035124:142404-142465 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin