Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1825868..1826370 Replicon chromosome
Accession NZ_CP035123
Organism Escherichia coli strain EC25

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EQK40_RS08765 Protein ID WP_000767829.1
Coordinates 1826116..1826370 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EQK40_RS08760 Protein ID WP_001259255.1
Coordinates 1825868..1826119 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EQK40_RS08735 1821045..1822112 - 1068 WP_000080093.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
EQK40_RS08740 1822112..1823182 - 1071 WP_053908449.1 histidinol-phosphate transaminase -
EQK40_RS08745 1823179..1824483 - 1305 WP_053908451.1 histidinol dehydrogenase -
EQK40_RS08750 1824489..1825388 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
EQK40_RS08755 1825534..1825584 - 51 WP_001364200.1 his operon leader peptide -
EQK40_RS08760 1825868..1826119 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EQK40_RS08765 1826116..1826370 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EQK40_RS08770 1826453..1827277 + 825 WP_053908453.1 SDR family oxidoreductase -
EQK40_RS08775 1827323..1828252 + 930 WP_053908455.1 LysR family transcriptional regulator -
EQK40_RS08780 1828467..1828529 + 63 WP_010723108.1 membrane protein YoeI -
EQK40_RS08785 1828519..1829877 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EQK40_RS08790 1830056..1831114 + 1059 WP_053908457.1 transport protein YeeE -
EQK40_RS08795 1831128..1831355 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T117216 WP_000767829.1 NZ_CP035123:1826116-1826370 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T117216 NZ_CP035123:1826116-1826370 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT117216 WP_001259255.1 NZ_CP035123:1825868-1826119 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT117216 NZ_CP035123:1825868-1826119 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References