Detailed information of TA system TA117175

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 26228..26886
• Replicon: plasmid p74.1Kbp
• Accession: NZ_CP035052
• Organism: Acinetobacter baumannii strain ABUH763

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: EP552_RS19290
• Protein ID: WP_000312250.1
• Coordinates: 26527..26886 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EP552_RS19285
• Protein ID: WP_001096429.1
• Coordinates: 26228..26527 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EP552_RS19240	22080..22532	+	453	Protein_31	hypothetical protein	-
EP552_RS19245	22637..22894	+	258	WP_000834290.1	hypothetical protein	-
EP552_RS19250	22899..23471	+	573	WP_000443897.1	hypothetical protein	-
EP552_RS19255	23447..23626	+	180	WP_000387630.1	hypothetical protein	-
EP552_RS19260	23643..24272	+	630	WP_000701004.1	hypothetical protein	-
EP552_RS19265	24312..24530	+	219	WP_001043201.1	hypothetical protein	-
EP552_RS19270	24550..25104	+	555	WP_000790084.1	hypothetical protein	-
EP552_RS19275	25154..25690	+	537	WP_000731978.1	hypothetical protein	-
EP552_RS19285	26228..26527	-	300	WP_001096429.1	helix-turn-helix transcriptional regulator	Antitoxin
EP552_RS19290	26527..26886	-	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EP552_RS19295	27087..27653	+	567	WP_000710385.1	hypothetical protein	-
EP552_RS19300	27702..27884	+	183	WP_000373385.1	hypothetical protein	-
EP552_RS19305	27951..28649	+	699	WP_000873188.1	hypothetical protein	-
EP552_RS19310	28788..29171	+	384	WP_000654348.1	hypothetical protein	-
EP552_RS19315	29238..29996	+	759	WP_001053127.1	hypothetical protein	-
EP552_RS19325	31051..31365	+	315	WP_000708714.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaOXA-23 / aph(3')-VIa	-	1..74091	74091

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T117175 WP_000312250.1 NZ_CP035052:c26886-26527 [Acinetobacter baumannii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T117175 NZ_CP035052:c26886-26527 [Acinetobacter baumannii]
ATGGCTTGGGATGTAGAAACAACAGAACTTTTTGATTCTTGGTTGGCAGAACAAGATGAAAATGCTCAAGATAAAATCTT
AGCGTCTTTGCTTGTTTTGTCAGAACTGGGGCCAAATCTAGGCCGGCCTCATGTTGATACTATTAAAGAATCAAAATATC
CGAACATGAAAGAGATACGTGTGCAAGTTAAAGGTCATCCGATCCGTGGTTTCTTTGCATTTGATCCAGAACGTAAAGCC
ATTGTTTTGTGTGCTGGAGATAAGAAAGGGCTAAATGAAAAAGCCTTTTATAAAGAAATGATCAAGATTGCAGATGAACA
ATATGAGCAATATTTACGTGATAATTACGGAGATAAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT117175 WP_001096429.1 NZ_CP035052:c26527-26228 [Acinetobacter baumannii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT117175 NZ_CP035052:c26527-26228 [Acinetobacter baumannii]
ATGGCTAAATTAAACGATATAATTTCAAAACGCTCACCTGAAAGCCAACAACGCATCAAGAAAATGGCAAATCAAATGCT
ATTGGATATTAACCTATCGAACCTACGGGAAGAGTTAAAAATATCTCAAGCTGAAATTGCAAAAAATCTAGGTGTAAGCC
AAGCTGCTATTTCAAAACGTGAAAAATTAGGTAAAGATCTTAAGATTTCATCATTAAAAGAATATGTTGAAGCTATGGGT
GGCAAAGTAAGGTTAGATGTTGAGTTACCTAACGGAAAGCATGTTGCTATATCTATTTAA