Detailed information of TA system TA117171

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 5911..6496
• Replicon: plasmid p11.8Kbp
• Accession: NZ_CP035050
• Organism: Acinetobacter baumannii strain ABUH773

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8ZPD7
• Locus tag: EQH48_RS18705
• Protein ID: WP_000897309.1
• Coordinates: 6176..6496 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EQH48_RS18700
• Protein ID: WP_000369780.1
• Coordinates: 5911..6183 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EQH48_RS18670	944..1519	+	576	WP_001096611.1	helix-turn-helix domain-containing protein	-
EQH48_RS18830	1541..1684	+	144	WP_000734097.1	hypothetical protein	-
EQH48_RS18675	1991..2266	+	276	WP_001133626.1	metal/formaldehyde-sensitive transcriptional repressor	-
EQH48_RS18680	2288..3397	+	1110	WP_000842148.1	S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase	-
EQH48_RS18685	3450..3848	+	399	WP_000235176.1	VOC family protein	-
EQH48_RS18695	4322..5155	+	834	WP_000439570.1	S-formylglutathione hydrolase	-
EQH48_RS18700	5911..6183	-	273	WP_000369780.1	helix-turn-helix domain-containing protein	Antitoxin
EQH48_RS18705	6176..6496	-	321	WP_000897309.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EQH48_RS18710	6683..6880	-	198	WP_000476222.1	hypothetical protein	-
EQH48_RS18715	6947..7174	-	228	WP_000921865.1	hypothetical protein	-
EQH48_RS18720	7273..7488	-	216	WP_000071892.1	cold-shock protein	-
EQH48_RS18725	7933..8958	-	1026	WP_001988464.1	IS30-like element ISAba125 family transposase	-
EQH48_RS18730	9003..9335	-	333	WP_000390243.1	hypothetical protein	-
EQH48_RS18735	9353..9619	-	267	WP_050441450.1	hypothetical protein	-
EQH48_RS18740	9829..11265	+	1437	WP_001029315.1	MobA/MobL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..11810	11810
	-	flank	IS/Tn	-	-	7933..8958	1025

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12209.01 Da
• Isoelectric Point: 7.9835

>T117171 WP_000897309.1 NZ_CP035050:c6496-6176 [Acinetobacter baumannii]
MLFIETSIFTKQIKELVSDEEYRQLQQDLLVQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETSILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T117171 NZ_CP035050:c6496-6176 [Acinetobacter baumannii]
ATGTTATTCATTGAAACCAGTATTTTTACTAAGCAAATCAAAGAGCTTGTGAGTGATGAAGAGTATCGTCAGCTCCAGCA
AGATCTCTTGGTACAGCCTGATAAGGGCGACTTAATCAAGAATGGTGGTGGAATTCGTAAAGTACGTTGTGCTCAGGGCA
ATAAAGGCAAAAGTGGCGGTATCCGTGTGATCTATTATTGGGTTACAGAAGATGATCAGATCTTTTTCCTGGTGGCTTAT
CCAAAGTCTGTGAAAGATAATCTGACAGACAAAGAAACCTCGATTCTACGTCAATTAGTAAAGGAGCAATTTCATGGATA
A

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10122.63 Da
• Isoelectric Point: 8.6582

>AT117171 WP_000369780.1 NZ_CP035050:c6183-5911 [Acinetobacter baumannii]
MDNNLFDDLVASIKEAGAIKRKEIKASRVTELELPDIKEVREKTGLTQAEFAARLHISARTLQNWEQGRRYPTGPAATLI
RILDAHPTLI

Nucleotide

• Length: 273 bp

>AT117171 NZ_CP035050:c6183-5911 [Acinetobacter baumannii]
ATGGATAACAACTTATTTGATGACCTGGTTGCTTCAATCAAAGAAGCTGGTGCCATCAAGCGTAAGGAGATAAAGGCAAG
TCGAGTCACAGAGCTTGAATTACCGGATATTAAAGAAGTACGTGAGAAAACAGGCTTAACCCAGGCTGAATTTGCTGCAC
GTTTGCATATCAGCGCGAGAACCCTGCAAAACTGGGAACAAGGCCGTCGCTATCCGACTGGTCCTGCAGCGACTTTAATT
CGTATTTTAGATGCTCATCCAACTTTAATTTAA

Structures

Toxin

• AlphaFold DB: A0A855WJQ3