117107

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-DinJ
Location 1803153..1803717 Replicon chromosome
Accession NZ_CP034973
Organism Lacticaseibacillus paracasei strain ALAC-4

Toxin (Protein)


Gene name yafQ Uniprot ID A0A510WIJ9
Locus tag EOK76_RS08480 Protein ID WP_016363448.1
Coordinates 1803153..1803455 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID K6SLT0
Locus tag EOK76_RS08485 Protein ID WP_003570051.1
Coordinates 1803448..1803717 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EOK76_RS08460 (EOK76_g1794) 1798836..1799273 + 438 WP_016385305.1 hypothetical protein -
EOK76_RS08465 (EOK76_g1795) 1799448..1801625 - 2178 WP_016385304.1 Tex family protein -
EOK76_RS08470 (EOK76_g1796) 1801861..1802553 - 693 WP_003570055.1 hypothetical protein -
EOK76_RS08475 (EOK76_g1797) 1802671..1802814 - 144 WP_162259784.1 hypothetical protein -
EOK76_RS08480 (EOK76_g1798) 1803153..1803455 - 303 WP_016363448.1 type II toxin-antitoxin system YafQ family toxin Toxin
EOK76_RS08485 (EOK76_g1799) 1803448..1803717 - 270 WP_003570051.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
EOK76_RS08490 (EOK76_g1800) 1804091..1804744 - 654 WP_003570049.1 N-acetyltransferase -
EOK76_RS08495 (EOK76_g1801) 1804789..1805418 - 630 WP_003570047.1 DUF2399 domain-containing protein -
EOK76_RS08500 (EOK76_g1802) 1805481..1805807 - 327 WP_003584272.1 YrdB family protein -
EOK76_RS08505 (EOK76_g1803) 1805800..1806204 - 405 WP_003570045.1 MerR family transcriptional regulator -
EOK76_RS08510 (EOK76_g1804) 1806402..1807628 + 1227 WP_003570043.1 hypothetical protein -
EOK76_RS08515 (EOK76_g1805) 1807825..1808712 + 888 WP_016385537.1 ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-97)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(10-70)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11510.33 Da        Isoelectric Point: 9.8881

>T117107 WP_016363448.1 NZ_CP034973:c1803455-1803153 [Lacticaseibacillus paracasei]
MYSLVPTPTFKRDLKRLSKKHWPMDELKTAVNLLAAGTNAELLSKKYADHALSSSSEWKGYRELYVDGPRGDWLLIYKIE
QQDLILTLVRTGSHHNLLVK

Download         Length: 303 bp

>T117107 NZ_CP034973:c1803455-1803153 [Lacticaseibacillus paracasei]
ATGTATAGTCTGGTTCCGACGCCTACATTTAAGCGCGATCTAAAACGACTCTCCAAGAAGCATTGGCCGATGGACGAACT
AAAGACGGCTGTTAATCTCCTAGCCGCTGGTACAAATGCTGAACTATTAAGCAAAAAGTATGCAGATCATGCCTTGTCCT
CAAGCAGCGAGTGGAAAGGATATCGTGAACTATATGTTGACGGCCCTCGTGGCGACTGGTTGCTAATCTATAAAATTGAG
CAGCAAGATCTTATTTTAACCCTAGTTAGAACCGGATCTCATCATAACCTTCTGGTTAAATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9982.68 Da        Isoelectric Point: 7.2026

>AT117107 WP_003570051.1 NZ_CP034973:c1803717-1803448 [Lacticaseibacillus paracasei]
MAATKKETRLNIRVDPELKSAAQIVANDMGIDLTAAVTMFMTKMVKDHALPFTPTSLPVETLQALKEAKHPELLKKYSTP
DDMWRDLNV

Download         Length: 270 bp

>AT117107 NZ_CP034973:c1803717-1803448 [Lacticaseibacillus paracasei]
ATGGCAGCCACAAAGAAGGAAACTCGCTTGAATATTCGTGTTGATCCTGAATTAAAAAGTGCTGCTCAAATCGTAGCAAA
TGATATGGGCATCGACTTGACCGCAGCTGTTACGATGTTCATGACGAAAATGGTGAAAGATCACGCCCTCCCGTTTACCC
CAACAAGTCTACCAGTTGAAACCTTACAGGCGCTGAAAGAAGCAAAGCACCCAGAGCTGCTCAAAAAATACAGCACGCCT
GATGACATGTGGAGAGACTTGAATGTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A510WIJ9

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BRK6

References