Detailed information of TA system TA117091

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1097317..1098044
• Replicon: chromosome
• Accession: NZ_CP034966
• Organism: Escherichia coli strain WCHEC020032

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: C7V17_RS07220
• Protein ID: WP_000547564.1
• Coordinates: 1097317..1097628 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C7V17_RS07225
• Protein ID: WP_000126294.1
• Coordinates: 1097625..1098044 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7V17_RS07190	1092459..1094168	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
C7V17_RS07195	1094178..1094720	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
C7V17_RS07200	1094720..1095487	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
C7V17_RS07205	1095484..1095894	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
C7V17_RS07210	1095887..1096357	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
C7V17_RS07215	1096382..1097143	+	762	WP_001026446.1	hypothetical protein	-
C7V17_RS07220	1097317..1097628	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
C7V17_RS07225	1097625..1098044	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
C7V17_RS07230	1098158..1099582	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
C7V17_RS07235	1099591..1101048	-	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
C7V17_RS07240	1101308..1102318	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
C7V17_RS07245	1102467..1102994	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T117091 WP_000547564.1 NZ_CP034966:1097317-1097628 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T117091 NZ_CP034966:1097317-1097628 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT117091 WP_000126294.1 NZ_CP034966:1097625-1098044 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT117091 NZ_CP034966:1097625-1098044 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5