Detailed information of TA system TA117082

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 46532..46802
• Replicon: plasmid pCTXM3_020032
• Accession: NZ_CP034964
• Organism: Escherichia coli strain WCHEC020032

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: C7V17_RS01245
• Protein ID: WP_001312861.1
• Coordinates: 46644..46802 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	46532..46595 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7V17_RS01220	42243..42770	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
C7V17_RS01225	42828..43061	+	234	WP_000006003.1	DUF905 family protein	-
C7V17_RS01230	43122..45145	+	2024	Protein_59	ParB/RepB/Spo0J family partition protein	-
C7V17_RS01235	45214..45648	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
C7V17_RS01240	45645..46364	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	46376..46600	+	225	NuclAT_0	-	-
-	46376..46600	+	225	NuclAT_0	-	-
-	46376..46600	+	225	NuclAT_0	-	-
-	46376..46600	+	225	NuclAT_0	-	-
C7V17_RS24700	46385..46564	-	180	WP_001309233.1	hypothetical protein	-
-	46532..46595	-	64	-	-	Antitoxin
C7V17_RS01245	46644..46802	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
C7V17_RS24880	47040..47417	-	378	Protein_64	hypothetical protein	-
C7V17_RS01265	47717..48013	+	297	WP_001272251.1	hypothetical protein	-
C7V17_RS01270	48124..48945	+	822	WP_001234445.1	DUF945 domain-containing protein	-
C7V17_RS01275	49242..49844	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
C7V17_RS01285	50167..50550	+	384	WP_001354030.1	relaxosome protein TraM	-
C7V17_RS01290	50744..51415	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
C7V17_RS01295	51552..51779	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / aac(3)-IId / mph(A) / sul1 / qacE / aadA5 / blaTEM-1B / blaCTX-M-3	-	1..87704	87704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T117082 WP_001312861.1 NZ_CP034964:46644-46802 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T117082 NZ_CP034964:46644-46802 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT117082 NZ_CP034964:c46595-46532 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin