Detailed information of TA system TA117078

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 33383..33647
• Replicon: plasmid pCMY42_020032
• Accession: NZ_CP034963
• Organism: Escherichia coli strain WCHEC020032

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Z417
• Locus tag: C7V17_RS00805
• Protein ID: WP_001387489.1
• Coordinates: 33383..33535 (-)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	33587..33647 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7V17_RS00775	28475..29008	-	534	WP_001221666.1	lipocalin family protein	-
C7V17_RS00780	29102..30247	-	1146	WP_039023136.1	class C extended-spectrum beta-lactamase CMY-42	-
C7V17_RS00785	30700..31397	-	698	Protein_32	IS1 family transposase	-
C7V17_RS00790	31622..31831	-	210	WP_000062603.1	HEAT repeat domain-containing protein	-
C7V17_RS24690	32223..32399	+	177	WP_001054897.1	hypothetical protein	-
C7V17_RS00795	32464..32760	-	297	WP_011264046.1	hypothetical protein	-
C7V17_RS00800	33060..33311	+	252	WP_001291964.1	hypothetical protein	-
C7V17_RS00805	33383..33535	-	153	WP_001387489.1	Hok/Gef family protein	Toxin
-	33587..33647	+	61	NuclAT_0	-	Antitoxin
-	33587..33647	+	61	NuclAT_0	-	Antitoxin
-	33587..33647	+	61	NuclAT_0	-	Antitoxin
-	33587..33647	+	61	NuclAT_0	-	Antitoxin
C7V17_RS00810	33850..34197	+	348	Protein_38	protein finQ	-
C7V17_RS00815	34383..35540	+	1158	Protein_39	IS1380-like element ISEc9 family transposase	-
C7V17_RS00820	35596..36293	+	698	Protein_40	IS1 family transposase	-
C7V17_RS00825	36307..36612	-	306	Protein_41	transcription termination factor NusG	-
C7V17_RS00835	37054..37341	-	288	WP_000074855.1	conjugal transfer protein TraA	-
C7V17_RS00840	37259..37522	-	264	WP_032180564.1	ash family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaCMY-42	-	1..38448	38448
	-	inside	IScluster/Tn	blaCMY-42	-	29102..35871	6769

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5761.10 Da
• Isoelectric Point: 8.7948

>T117078 WP_001387489.1 NZ_CP034963:c33535-33383 [Escherichia coli]
MPQRTFLMMLIVVCVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T117078 NZ_CP034963:c33535-33383 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT117078 NZ_CP034963:33587-33647 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: U9Z417

Antitoxin