Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1843148..1843650 Replicon chromosome
Accession NZ_CP034958
Organism Escherichia coli strain SCEC020026

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag C7V14_RS09835 Protein ID WP_000767829.1
Coordinates 1843396..1843650 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag C7V14_RS09830 Protein ID WP_001259255.1
Coordinates 1843148..1843399 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C7V14_RS09805 1838325..1839392 - 1068 WP_000080060.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
C7V14_RS09810 1839392..1840462 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
C7V14_RS09815 1840459..1841763 - 1305 WP_001317939.1 histidinol dehydrogenase -
C7V14_RS09820 1841769..1842668 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
C7V14_RS09825 1842814..1842864 - 51 WP_001364200.1 his operon leader peptide -
C7V14_RS09830 1843148..1843399 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
C7V14_RS09835 1843396..1843650 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
C7V14_RS09840 1843733..1844557 + 825 WP_000754758.1 SDR family oxidoreductase -
C7V14_RS09845 1844603..1845532 + 930 WP_000803351.1 LysR family transcriptional regulator -
C7V14_RS09850 1845747..1845809 + 63 WP_010723108.1 membrane protein YoeI -
C7V14_RS09855 1845799..1847157 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
C7V14_RS09860 1847336..1848394 + 1059 WP_000492346.1 transport protein YeeE -
C7V14_RS09865 1848408..1848635 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T117063 WP_000767829.1 NZ_CP034958:1843396-1843650 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T117063 NZ_CP034958:1843396-1843650 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT117063 WP_001259255.1 NZ_CP034958:1843148-1843399 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT117063 NZ_CP034958:1843148-1843399 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References