Detailed information of TA system TA117061

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1111493..1112220
• Replicon: chromosome
• Accession: NZ_CP034958
• Organism: Escherichia coli strain SCEC020026

Toxin (Protein)

• Gene name: higB
• Uniprot ID: J7Q991
• Locus tag: C7V14_RS06490
• Protein ID: WP_000547564.1
• Coordinates: 1111493..1111804 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C7V14_RS06495
• Protein ID: WP_000126294.1
• Coordinates: 1111801..1112220 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7V14_RS06460	1106635..1108344	+	1710	WP_001288134.1	formate hydrogenlyase subunit HycE	-
C7V14_RS06465	1108354..1108896	+	543	WP_000493792.1	formate hydrogenlyase subunit HycF	-
C7V14_RS06470	1108896..1109663	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
C7V14_RS06475	1109660..1110070	+	411	WP_001291921.1	formate hydrogenlyase assembly protein HycH	-
C7V14_RS06480	1110063..1110533	+	471	WP_060621146.1	hydrogenase maturation peptidase HycI	-
C7V14_RS06485	1110558..1111319	+	762	WP_001026446.1	hypothetical protein	-
C7V14_RS06490	1111493..1111804	+	312	WP_000547564.1	type II toxin-antitoxin system HigB family toxin	Toxin
C7V14_RS06495	1111801..1112220	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
C7V14_RS06500	1112334..1113758	-	1425	WP_000110363.1	6-phospho-beta-glucosidase AscB	-
C7V14_RS06505	1113767..1115224	-	1458	WP_001107861.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
C7V14_RS06510	1115484..1116494	+	1011	WP_001392554.1	DNA-binding transcriptional regulator AscG	-
C7V14_RS06515	1116643..1117170	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12426.13 Da
• Isoelectric Point: 9.7248

>T117061 WP_000547564.1 NZ_CP034958:1111493-1111804 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T117061 NZ_CP034958:1111493-1111804 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT117061 WP_000126294.1 NZ_CP034958:1111801-1112220 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT117061 NZ_CP034958:1111801-1112220 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A0A0FJG5