Detailed information of TA system TA117049

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 58585..58824
• Replicon: plasmid pCTXM15_020026
• Accession: NZ_CP034956
• Organism: Escherichia coli strain SCEC020026

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: C7V14_RS00455
• Protein ID: WP_023144756.1
• Coordinates: 58585..58719 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	58764..58824 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C7V14_RS00425	54286..55143	-	858	WP_000130640.1	incFII family plasmid replication initiator RepA	-
C7V14_RS00430	55136..55210	-	75	WP_031943482.1	RepA leader peptide Tap	-
C7V14_RS24305	55207..55341	-	135	Protein_67	protein CopA/IncA	-
C7V14_RS00440	55572..57113	+	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
C7V14_RS00445	57128..57874	+	747	WP_053906593.1	IS21-like element ISEc12 family helper ATPase IstB	-
C7V14_RS00450	58034..58288	-	255	WP_000083850.1	replication regulatory protein RepA	-
C7V14_RS00455	58585..58719	-	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	58764..58824	+	61	NuclAT_0	-	Antitoxin
-	58764..58824	+	61	NuclAT_0	-	Antitoxin
-	58764..58824	+	61	NuclAT_0	-	Antitoxin
-	58764..58824	+	61	NuclAT_0	-	Antitoxin
C7V14_RS24160	58791..59077	-	287	Protein_72	DUF2726 domain-containing protein	-
C7V14_RS00470	59590..59802	-	213	WP_013023861.1	hypothetical protein	-
C7V14_RS00475	59933..60493	-	561	WP_000139341.1	fertility inhibition protein FinO	-
C7V14_RS00480	60548..61294	-	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-
C7V14_RS00485	61314..63707	-	2394	Protein_76	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(B) / sul1 / qacE / aadA5 / mph(A) / aac(3)-IId / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr	-	1..98397	98397

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T117049 WP_023144756.1 NZ_CP034956:c58719-58585 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T117049 NZ_CP034956:c58719-58585 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTTGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACAGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT117049 NZ_CP034956:58764-58824 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin