Detailed information of TA system TA117044

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 3850319..3850731
• Replicon: chromosome
• Accession: NZ_CP034953
• Organism: Escherichia coli strain MT102

Toxin (Protein)

• Gene name: symE
• Uniprot ID: U9YSY7
• Locus tag: EPB33_RS18765
• Protein ID: WP_000132601.1
• Coordinates: 3850390..3850731 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	3850319..3850395 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EPB33_RS18755	3847182..3848771	+	1590	WP_001063204.1	type I restriction-modification system methyltransferase	-
EPB33_RS18760	3848768..3850162	+	1395	WP_001272447.1	type I restriction-modification system specificity subunit	-
-	3850319..3850395	-	77	NuclAT_14	-	Antitoxin
-	3850319..3850395	-	77	NuclAT_14	-	Antitoxin
-	3850319..3850395	-	77	NuclAT_14	-	Antitoxin
-	3850319..3850395	-	77	NuclAT_14	-	Antitoxin
-	3850319..3850395	-	77	NuclAT_15	-	Antitoxin
-	3850319..3850395	-	77	NuclAT_15	-	Antitoxin
-	3850319..3850395	-	77	NuclAT_15	-	Antitoxin
-	3850319..3850395	-	77	NuclAT_15	-	Antitoxin
EPB33_RS18765	3850390..3850731	+	342	WP_000132601.1	endoribonuclease SymE	Toxin
EPB33_RS18770	3850893..3852272	+	1380	Protein_3660	5-methylcytosine-specific restriction endonuclease subunit McrB	-
EPB33_RS18775	3852272..3853318	+	1047	WP_000437621.1	5-methylcytosine-specific restriction endonuclease system specificity protein McrC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12203.02 Da
• Isoelectric Point: 8.5012

>T117044 WP_000132601.1 NZ_CP034953:3850390-3850731 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRHVTVGYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQAFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T117044 NZ_CP034953:3850390-3850731 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin

Nucleotide

• Length: 77 bp

>AT117044 NZ_CP034953:c3850395-3850319 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: U9YSY7

Antitoxin