Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1791352..1791854 Replicon chromosome
Accession NZ_CP034953
Organism Escherichia coli strain MT102

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag EPB33_RS08500 Protein ID WP_000767829.1
Coordinates 1791600..1791854 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag EPB33_RS08495 Protein ID WP_001259255.1
Coordinates 1791352..1791603 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EPB33_RS08470 1786530..1787597 - 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
EPB33_RS08475 1787597..1788667 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
EPB33_RS08480 1788664..1789968 - 1305 WP_000009594.1 histidinol dehydrogenase -
EPB33_RS08485 1789974..1790873 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
EPB33_RS08490 1791019..1791069 - 51 WP_001364200.1 his operon leader peptide -
EPB33_RS08495 1791352..1791603 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
EPB33_RS08500 1791600..1791854 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
EPB33_RS08505 1791937..1792761 + 825 WP_000754737.1 SDR family oxidoreductase -
EPB33_RS08510 1792807..1793736 + 930 WP_000803351.1 LysR family transcriptional regulator -
EPB33_RS08515 1793951..1794013 + 63 WP_010723108.1 membrane protein YoeI -
EPB33_RS08520 1794003..1795361 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
EPB33_RS08525 1795540..1796598 + 1059 WP_000492339.1 transport protein YeeE -
EPB33_RS08530 1796612..1796839 + 228 WP_000234896.1 sulfurtransferase TusA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T117027 WP_000767829.1 NZ_CP034953:1791600-1791854 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T117027 NZ_CP034953:1791600-1791854 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT117027 WP_001259255.1 NZ_CP034953:1791352-1791603 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT117027 NZ_CP034953:1791352-1791603 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References