Detailed information of TA system TA117004

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HTH_XRE
• Location: 3713355..3714089
• Replicon: chromosome
• Accession: NZ_CP034938
• Organism: Pectobacterium odoriferum strain JK2.1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: EO763_RS16760
• Protein ID: WP_103161045.1
• Coordinates: 3713775..3714089 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EO763_RS16755
• Protein ID: WP_103161044.1
• Coordinates: 3713355..3713696 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EO763_RS16710	3708609..3709736	+	1128	WP_161529624.1	phage major capsid protein, P2 family	-
EO763_RS16715	3709740..3710399	+	660	WP_161529625.1	terminase endonuclease subunit	-
EO763_RS16720	3710491..3710994	+	504	WP_161529626.1	head completion/stabilization protein	-
EO763_RS16725	3710994..3711197	+	204	WP_161529627.1	tail protein X	-
EO763_RS16730	3711188..3711406	+	219	WP_039537277.1	hypothetical protein	-
EO763_RS16735	3711390..3711899	+	510	WP_161529628.1	lysozyme	-
EO763_RS16740	3711896..3712339	+	444	WP_161529629.1	Rz-like lysis system protein LysB	-
EO763_RS16745	3712429..3712884	+	456	WP_161529630.1	phage tail protein	-
EO763_RS16750	3712877..3713326	+	450	WP_161529631.1	phage virion morphogenesis protein	-
EO763_RS16755	3713355..3713696	-	342	WP_103161044.1	HigA family addiction module antidote protein	Antitoxin
EO763_RS16760	3713775..3714089	-	315	WP_103161045.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EO763_RS16765	3714433..3715071	+	639	WP_103161046.1	ORF6N domain-containing protein	-
EO763_RS16770	3715116..3716174	-	1059	WP_103161047.1	hypothetical protein	-
EO763_RS16775	3716273..3716914	+	642	WP_161529632.1	phage baseplate assembly protein V	-
EO763_RS16780	3716911..3717255	+	345	WP_039475889.1	GPW/gp25 family protein	-
EO763_RS16785	3717260..3718168	+	909	WP_161529633.1	baseplate assembly protein	-
EO763_RS16790	3718161..3718772	+	612	WP_161529634.1	phage tail protein I	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	luxS	3693543..3749026	55483

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (9-98)

Antitoxin

• Cro/C1-type helix-turn-helix domain (24-70)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12256.06 Da
• Isoelectric Point: 9.9719

>T117004 WP_103161045.1 NZ_CP034938:c3714089-3713775 [Pectobacterium odoriferum]
MQKMRSIHSFRDQWLEDFFMYGKSHKKIPVDVVTSLSRKLDIINAAVSAKDLRSPPGNRYENLNPPLGEYSSIRVNIQYR
LIFKWVDGKAEDIYLDDHGYKKHK

Nucleotide

• Length: 315 bp

>T117004 NZ_CP034938:c3714089-3713775 [Pectobacterium odoriferum]
ATGCAAAAAATGCGCAGCATTCATAGCTTTCGGGATCAGTGGCTGGAAGATTTTTTTATGTATGGCAAGTCGCATAAAAA
GATACCTGTCGATGTGGTTACATCCTTGTCCCGCAAGCTGGATATCATCAACGCGGCGGTTTCAGCAAAAGACCTTAGAT
CGCCGCCGGGGAACCGATACGAGAACCTTAACCCACCATTGGGGGAATATTCATCCATCAGAGTAAATATCCAATATCGC
CTGATTTTTAAATGGGTTGATGGTAAAGCAGAGGATATCTACCTTGACGACCATGGTTATAAAAAGCACAAATAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12942.54 Da
• Isoelectric Point: 4.3501

>AT117004 WP_103161044.1 NZ_CP034938:c3713696-3713355 [Pectobacterium odoriferum]
MAQAQRKPTSVGDVLLYEYLEPTGLKINDLAEMLNVHRNTISALVNNNRKLTVDMAFRLAKAFDTSVDFWLNLQNSVDVW
EVENDARTQEEISRITTLEDFLAQRDENVKDVA

Nucleotide

• Length: 342 bp

>AT117004 NZ_CP034938:c3713696-3713355 [Pectobacterium odoriferum]
ATGGCACAGGCACAACGCAAGCCGACATCCGTAGGCGATGTCTTGTTATATGAATATCTAGAACCTACAGGTTTGAAGAT
TAATGACTTGGCGGAAATGCTGAATGTCCACCGCAACACGATAAGCGCACTGGTCAATAACAACCGTAAGTTAACGGTTG
ATATGGCGTTCCGGTTGGCGAAAGCATTTGATACGTCAGTTGATTTTTGGCTGAACCTGCAAAATAGTGTTGACGTGTGG
GAAGTAGAAAACGACGCACGTACTCAGGAAGAAATTAGTCGGATTACTACGCTGGAAGATTTTCTGGCGCAGCGTGATGA
AAATGTGAAAGACGTAGCGTGA