Detailed information of TA system TA116958

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 65566..65992
• Replicon: plasmid p2013C-3749-1
• Accession: NZ_CP034932
• Organism: Shigella flexneri strain 2013C-3749

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: EPD86_RS23930
• Protein ID: WP_001372321.1
• Coordinates: 65566..65691 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	65768..65992 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EPD86_RS23890 (60589)	60589..60816	-	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-
EPD86_RS23895 (60953)	60953..61624	-	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
EPD86_RS23900 (61818)	61818..62201	-	384	WP_001354030.1	conjugal transfer relaxosome DNA-binding protein TraM	-
EPD86_RS23905 (62536)	62536..63126	+	591	WP_000252683.1	transglycosylase SLT domain-containing protein	-
EPD86_RS23910 (63423)	63423..64244	-	822	WP_001234445.1	DUF932 domain-containing protein	-
EPD86_RS23915 (64355)	64355..64651	-	297	WP_001272251.1	hypothetical protein	-
EPD86_RS26015 (64951)	64951..65247	+	297	Protein_79	hypothetical protein	-
EPD86_RS23930 (65566)	65566..65691	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
EPD86_RS26020 (65633)	65633..65782	-	150	Protein_81	plasmid maintenance protein Mok	-
EPD86_RS23935 (65804)	65804..65983	+	180	WP_001309233.1	hypothetical protein	-
- (65768)	65768..65992	-	225	NuclAT_0	-	Antitoxin
- (65768)	65768..65992	-	225	NuclAT_0	-	Antitoxin
- (65768)	65768..65992	-	225	NuclAT_0	-	Antitoxin
- (65768)	65768..65992	-	225	NuclAT_0	-	Antitoxin
EPD86_RS23940 (66004)	66004..66723	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
EPD86_RS23945 (66720)	66720..67154	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
EPD86_RS23950 (67223)	67223..69246	-	2024	Protein_85	ParB/RepB/Spo0J family partition protein	-
EPD86_RS23955 (69307)	69307..69540	-	234	WP_000006003.1	DUF905 family protein	-
EPD86_RS23960 (69598)	69598..70125	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
EPD86_RS26025 (70427)	70427..70882	+	456	Protein_88	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T116958 WP_001372321.1 NZ_CP034932:c65691-65566 [Shigella flexneri]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T116958 NZ_CP034932:c65691-65566 [Shigella flexneri]
GTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACGAAAATCGCTGTGCGAGATTCGTTACAGAGACGG
ACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT116958 NZ_CP034932:c65992-65768 [Shigella flexneri]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin