116939

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 639938..640556 Replicon chromosome
Accession NZ_CP034931
Organism Shigella flexneri strain 2013C-3749

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag EPD86_RS03180 Protein ID WP_001291435.1
Coordinates 639938..640156 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q0T7C3
Locus tag EPD86_RS03185 Protein ID WP_000344797.1
Coordinates 640182..640556 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EPD86_RS03150 (635830) 635830..636141 - 312 WP_000409911.1 MGMT family protein -
EPD86_RS03160 (636520) 636520..636873 + 354 WP_000878140.1 DUF1428 domain-containing protein -
EPD86_RS03165 (636915) 636915..638465 - 1551 WP_005098218.1 cyclic-guanylate-specific phosphodiesterase PdeB -
EPD86_RS03170 (638629) 638629..639099 - 471 WP_000136192.1 YlaC family protein -
EPD86_RS03175 (639215) 639215..639766 - 552 Protein_622 maltose O-acetyltransferase -
EPD86_RS03180 (639938) 639938..640156 - 219 WP_001291435.1 HHA domain-containing protein Toxin
EPD86_RS03185 (640182) 640182..640556 - 375 WP_000344797.1 Hha toxicity modulator TomB Antitoxin
EPD86_RS03190 (641102) 641102..644251 - 3150 WP_001132484.1 efflux RND transporter permease AcrB -
EPD86_RS03195 (644274) 644274..645467 - 1194 WP_011069267.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 634472..635800 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T116939 WP_001291435.1 NZ_CP034931:c640156-639938 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T116939 NZ_CP034931:c640156-639938 [Shigella flexneri]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14513.38 Da        Isoelectric Point: 4.9091

>AT116939 WP_000344797.1 NZ_CP034931:c640556-640182 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQALQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT116939 NZ_CP034931:c640556-640182 [Shigella flexneri]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGTTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGCTCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAACGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TPD8

References