Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 539910..540604 Replicon chromosome
Accession NZ_CP034931
Organism Shigella flexneri strain 2013C-3749

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag EPD86_RS02665 Protein ID WP_001263491.1
Coordinates 540206..540604 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID Q0T7Q4
Locus tag EPD86_RS02660 Protein ID WP_000554759.1
Coordinates 539910..540203 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EPD86_RS02640 (535549) 535549..536046 + 498 WP_000006251.1 REP-associated tyrosine transposase RayT -
EPD86_RS02645 (536263) 536263..537975 - 1713 Protein_519 flagellar biosynthesis protein FlhA -
EPD86_RS02650 (537947) 537947..538732 + 786 WP_000207560.1 putative lateral flagellar export/assembly protein LafU -
EPD86_RS02655 (538803) 538803..539858 + 1056 WP_001226172.1 DNA polymerase IV -
EPD86_RS02660 (539910) 539910..540203 + 294 WP_000554759.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
EPD86_RS02665 (540206) 540206..540604 + 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
EPD86_RS02670 (540614) 540614..541066 + 453 WP_001059860.1 GNAT family N-acetyltransferase -
EPD86_RS02675 (541312) 541312..542355 + 1044 WP_005053205.1 RNA ligase RtcB family protein -
EPD86_RS02680 (542471) 542471..543031 + 561 Protein_526 peptide chain release factor H -
EPD86_RS02685 (543088) 543088..544545 - 1458 WP_001293030.1 cytosol nonspecific dipeptidase -
EPD86_RS02690 (544807) 544807..545265 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T116937 WP_001263491.1 NZ_CP034931:540206-540604 [Shigella flexneri]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T116937 NZ_CP034931:540206-540604 [Shigella flexneri]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11232.82 Da        Isoelectric Point: 7.0153

>AT116937 WP_000554759.1 NZ_CP034931:539910-540203 [Shigella flexneri]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
KQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT116937 NZ_CP034931:539910-540203 [Shigella flexneri]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
AGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTTCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
AAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TR46

References