Detailed information of TA system TA116869

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2136546..2137300
• Replicon: chromosome
• Accession: NZ_CP034819
• Organism: Salmonella sp. SSDFZ54

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B5F003
• Locus tag: EOS97_RS10685
• Protein ID: WP_000558166.1
• Coordinates: 2136989..2137300 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EOS97_RS10680
• Protein ID: WP_001259011.1
• Coordinates: 2136546..2136992 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EOS97_RS10650	2131722..2132618	+	897	WP_001805206.1	sugar kinase	-
EOS97_RS10655	2132652..2133455	+	804	WP_000059693.1	DeoR/GlpR family DNA-binding transcription regulator	-
EOS97_RS10660	2133646..2134245	+	600	WP_000965695.1	glucose-1-phosphatase	-
EOS97_RS10665	2134239..2135111	+	873	WP_000921423.1	virulence factor BrkB family protein	-
EOS97_RS10670	2135108..2135545	+	438	WP_000560969.1	D-tyrosyl-tRNA(Tyr) deacylase	-
EOS97_RS10675	2135590..2136531	+	942	WP_001518251.1	fatty acid biosynthesis protein FabY	-
EOS97_RS10680	2136546..2136992	-	447	WP_001259011.1	type II toxin-antitoxin system HigA family antitoxin	Antitoxin
EOS97_RS10685	2136989..2137300	-	312	WP_000558166.1	type II toxin-antitoxin system HigB family toxin	Toxin
EOS97_RS10690	2137386..2138315	-	930	WP_001127701.1	alpha/beta hydrolase	-
EOS97_RS10695	2138533..2138844	+	312	WP_001159628.1	hypothetical protein	-
EOS97_RS10700	2138845..2139135	+	291	WP_000362050.1	DNA-binding transcriptional regulator	-
EOS97_RS10705	2139182..2140111	-	930	WP_000027730.1	formate dehydrogenase accessory protein FdhE	-
EOS97_RS10710	2140108..2140743	-	636	WP_000829025.1	formate dehydrogenase cytochrome b556 subunit	-
EOS97_RS10715	2140740..2141642	-	903	WP_000331361.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (93-146)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12432.40 Da
• Isoelectric Point: 9.5334

>T116869 WP_000558166.1 NZ_CP034819:c2137300-2136989 [Salmonella sp. SSDFZ54]
VHVISRKPFNEAMLMYPNHELALTELLNVLEKKTFTQPEEMKRYIPSLDNFKYRDKWWVIDVSGNSLRLISYIDFRLHKI
FVKHIVSHAEYDKLTAYYRGNKE

Nucleotide

• Length: 312 bp

>T116869 NZ_CP034819:c2137300-2136989 [Salmonella sp. SSDFZ54]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATATTATCGGGGTAATAAAGAATGA

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16734.08 Da
• Isoelectric Point: 6.6451

>AT116869 WP_001259011.1 NZ_CP034819:c2136992-2136546 [Salmonella sp. SSDFZ54]
MRTHRQMDATSAKKIVDTFSDAVKTVPLMGEDRNDNEYRRALALVEFLVDHDDLENPLFELLCARISEYEKHAPEFKALN
QHLEKTPPGVSVLRTLMDQYGLKAADLANELGSKSNVSNILNGRRALTVNHIKALTQRFKLPADAFIE

Nucleotide

• Length: 447 bp

>AT116869 NZ_CP034819:c2136992-2136546 [Salmonella sp. SSDFZ54]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTTAAACTACCAGCGGATGCCTTCATCGAGTAG

Structures

Toxin

• AlphaFold DB: A0A0R9ME90