Detailed information of TA system TA116833

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1032986..1033680
• Replicon: chromosome
• Accession: NZ_CP034808
• Organism: Escherichia coli strain 08-3914

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: AYP36_RS05005
• Protein ID: WP_001263495.1
• Coordinates: 1032986..1033384 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: AYP36_RS05010
• Protein ID: WP_000554757.1
• Coordinates: 1033387..1033680 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	1028573..1028653	-	81	NuclAT_11	-	-
-	1028573..1028653	-	81	NuclAT_11	-	-
-	1028573..1028653	-	81	NuclAT_11	-	-
-	1028573..1028653	-	81	NuclAT_11	-	-
AYP36_RS04980	1029249..1029707	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
AYP36_RS04985	1029968..1031425	+	1458	WP_001293020.1	cytosol nonspecific dipeptidase	-
AYP36_RS04990	1031482..1032003	-	522	Protein_939	peptide chain release factor H	-
AYP36_RS04995	1032002..1032205	-	204	Protein_940	RNA ligase RtcB family protein	-
AYP36_RS05000	1032524..1032976	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
AYP36_RS05005	1032986..1033384	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
AYP36_RS05010	1033387..1033680	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
AYP36_RS05015	1033732..1034787	-	1056	WP_001226186.1	DNA polymerase IV	-
AYP36_RS05020	1034858..1035643	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
AYP36_RS05025	1035615..1037327	+	1713	Protein_946	flagellar biosynthesis protein FlhA	-
AYP36_RS05030	1037472..1037969	-	498	Protein_947	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	994854..1038918	44064

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T116833 WP_001263495.1 NZ_CP034808:c1033384-1032986 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T116833 NZ_CP034808:c1033384-1032986 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT116833 WP_000554757.1 NZ_CP034808:c1033680-1033387 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT116833 NZ_CP034808:c1033680-1033387 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6