Detailed information of TA system TA116828

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 145252..145477
• Replicon: chromosome
• Accession: NZ_CP034808
• Organism: Escherichia coli strain 08-3914

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: -
• Locus tag: AYP36_RS00740
• Protein ID: WP_000813263.1
• Coordinates: 145252..145407 (-)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	145419..145477 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYP36_RS00705	140706..141419	-	714	WP_000261909.1	helix-turn-helix domain-containing protein	-
AYP36_RS00710	141557..141753	-	197	Protein_129	TrmB family transcriptional regulator	-
AYP36_RS00715	142040..142858	-	819	WP_000265265.1	CPBP family intramembrane metalloprotease	-
AYP36_RS00720	143010..143381	-	372	WP_000090264.1	antitermination protein	-
AYP36_RS00725	143371..143742	-	372	WP_001217436.1	RusA family crossover junction endodeoxyribonuclease	-
AYP36_RS00730	143755..144804	-	1050	WP_001265172.1	DUF968 domain-containing protein	-
AYP36_RS00735	144806..145084	-	279	WP_001341388.1	hypothetical protein	-
AYP36_RS00740	145252..145407	-	156	WP_000813263.1	type I toxin-antitoxin system toxin HokD	Toxin
-	145419..145477	+	59	-	-	Antitoxin
AYP36_RS00755	146012..146785	+	774	WP_000160654.1	alpha/beta hydrolase	-
AYP36_RS00760	147137..147550	-	414	WP_001151233.1	DUF977 family protein	-
AYP36_RS00765	147566..148336	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
AYP36_RS00770	148358..149104	-	747	WP_000788745.1	ATP-binding protein	-
AYP36_RS00775	149111..150202	-	1092	WP_001205823.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5722.86 Da
• Isoelectric Point: 6.1531

>T116828 WP_000813263.1 NZ_CP034808:c145407-145252 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T116828 NZ_CP034808:c145407-145252 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAGTCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT116828 NZ_CP034808:145419-145477 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTAGATTTGCCGTTCATAGATTGAGCCTCA

Structures

Antitoxin