Detailed information of TA system TA116797

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 678906..679600
• Replicon: chromosome
• Accession: NZ_CP034806
• Organism: Escherichia coli strain 2010C-3347

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: A1D75_RS03085
• Protein ID: WP_001263495.1
• Coordinates: 679202..679600 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: A1D75_RS03080
• Protein ID: WP_000554757.1
• Coordinates: 678906..679199 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A1D75_RS03060	674617..675114	+	498	Protein_596	REP-associated tyrosine transposase RayT	-
A1D75_RS03065	675259..676971	-	1713	Protein_597	flagellar biosynthesis protein FlhA	-
A1D75_RS03070	676943..677728	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
A1D75_RS03075	677799..678854	+	1056	WP_001226188.1	DNA polymerase IV	-
A1D75_RS03080	678906..679199	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
A1D75_RS03085	679202..679600	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
A1D75_RS03090	679610..680062	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
A1D75_RS03095	680381..680584	+	204	Protein_603	RNA ligase RtcB family protein	-
A1D75_RS03100	680583..681104	+	522	Protein_604	peptide chain release factor H	-
A1D75_RS03105	681161..682618	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
A1D75_RS03110	682879..683337	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	683933..684013	+	81	NuclAT_12	-	-
-	683933..684013	+	81	NuclAT_12	-	-
-	683933..684013	+	81	NuclAT_12	-	-
-	683933..684013	+	81	NuclAT_12	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T116797 WP_001263495.1 NZ_CP034806:679202-679600 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T116797 NZ_CP034806:679202-679600 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT116797 WP_000554757.1 NZ_CP034806:678906-679199 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT116797 NZ_CP034806:678906-679199 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6