Detailed information of TA system TA116787

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 65459..65723
• Replicon: plasmid p2009C-3554-1
• Accession: NZ_CP034804
• Organism: Escherichia coli strain 2009C-3554

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: A0I22_RS28575
• Protein ID: WP_001331364.1
• Coordinates: 65571..65723 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	65459..65521 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A0I22_RS28560	60698..62989	-	2292	WP_001289276.1	hypothetical protein	-
A0I22_RS28565	62982..64052	-	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
A0I22_RS28570	64071..65279	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	65459..65521	-	63	NuclAT_0	-	Antitoxin
-	65459..65521	-	63	NuclAT_0	-	Antitoxin
-	65459..65521	-	63	NuclAT_0	-	Antitoxin
-	65459..65521	-	63	NuclAT_0	-	Antitoxin
A0I22_RS28575	65571..65723	+	153	WP_001331364.1	Hok/Gef family protein	Toxin
A0I22_RS28580	65795..66046	-	252	WP_001291964.1	hypothetical protein	-
A0I22_RS29390	66705..66881	-	177	WP_001054897.1	hypothetical protein	-
A0I22_RS28585	67273..67482	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
A0I22_RS28590	67554..68204	-	651	WP_001178506.1	plasmid IncI1-type surface exclusion protein ExcA	-
A0I22_RS28595	68278..70446	-	2169	WP_000698357.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..83424	83424

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T116787 WP_001331364.1 NZ_CP034804:65571-65723 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T116787 NZ_CP034804:65571-65723 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT116787 NZ_CP034804:c65521-65459 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin