Detailed information of TA system TA116763

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1994118..1994812
• Replicon: chromosome
• Accession: NZ_CP034803
• Organism: Escherichia coli strain 2009C-3554

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: A0I22_RS10745
• Protein ID: WP_001263495.1
• Coordinates: 1994118..1994516 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: A0I22_RS10750
• Protein ID: WP_000554757.1
• Coordinates: 1994519..1994812 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	1989705..1989785	-	81	NuclAT_12	-	-
-	1989705..1989785	-	81	NuclAT_12	-	-
-	1989705..1989785	-	81	NuclAT_12	-	-
-	1989705..1989785	-	81	NuclAT_12	-	-
A0I22_RS10720	1990381..1990839	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
A0I22_RS10725	1991100..1992557	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
A0I22_RS10730	1992614..1993135	-	522	Protein_2016	peptide chain release factor H	-
A0I22_RS10735	1993134..1993337	-	204	Protein_2017	RNA ligase RtcB family protein	-
A0I22_RS10740	1993656..1994108	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
A0I22_RS10745	1994118..1994516	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
A0I22_RS10750	1994519..1994812	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
A0I22_RS10755	1994864..1995919	-	1056	WP_001226188.1	DNA polymerase IV	-
A0I22_RS10760	1995990..1996775	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
A0I22_RS10765	1996747..1998459	+	1713	Protein_2023	flagellar biosynthesis protein FlhA	-
A0I22_RS10770	1998604..1999101	-	498	Protein_2024	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T116763 WP_001263495.1 NZ_CP034803:c1994516-1994118 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T116763 NZ_CP034803:c1994516-1994118 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT116763 WP_000554757.1 NZ_CP034803:c1994812-1994519 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT116763 NZ_CP034803:c1994812-1994519 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6