Detailed information of TA system TA116754

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 190008..190379
• Replicon: chromosome
• Accession: NZ_CP034803
• Organism: Escherichia coli strain 2009C-3554

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A0D6ZRD3
• Locus tag: A0I22_RS01080
• Protein ID: WP_001443846.1
• Coordinates: 190230..190379 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	190008..190186 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A0I22_RS29240	185760..185930	+	171	WP_001625136.1	hypothetical protein	-
A0I22_RS01055	185963..187336	+	1374	WP_000123746.1	ATP-dependent RNA helicase DbpA	-
A0I22_RS01060	187465..188400	-	936	WP_001157407.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
A0I22_RS01065	188452..189687	-	1236	WP_000040839.1	site-specific integrase	-
A0I22_RS01070	189689..189904	-	216	WP_000079604.1	excisionase XisR	-
A0I22_RS01075	190004..190192	-	189	WP_001302840.1	DUF1187 family protein	-
-	190008..190186	+	179	NuclAT_0	-	Antitoxin
-	190008..190186	+	179	NuclAT_0	-	Antitoxin
-	190008..190186	+	179	NuclAT_0	-	Antitoxin
-	190008..190186	+	179	NuclAT_0	-	Antitoxin
A0I22_RS01080	190230..190379	-	150	WP_001443846.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
A0I22_RS01085	190435..191244	-	810	WP_000166313.1	recombination protein RecT	-
A0I22_RS01090	191237..193837	-	2601	WP_000105140.1	exodeoxyribonuclease VIII	-
A0I22_RS01095	193939..194214	-	276	WP_001344816.1	hypothetical protein	-
A0I22_RS01100	194289..194459	-	171	WP_001352098.1	conserved protein, Rac prophage	-
A0I22_RS01105	194459..194680	-	222	WP_000560223.1	killing protein KilR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	189689..205468	15779

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-48)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5383.12 Da
• Isoelectric Point: 8.3398

>T116754 WP_001443846.1 NZ_CP034803:c190379-190230 [Escherichia coli]
MRYDNVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALER

Nucleotide

• Length: 150 bp

>T116754 NZ_CP034803:c190379-190230 [Escherichia coli]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGA

Antitoxin

Nucleotide

• Length: 179 bp

>AT116754 NZ_CP034803:190008-190186 [Escherichia coli]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A0D6ZRD3

Antitoxin