Detailed information of TA system TA116748

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 4761673..4761898
• Replicon: chromosome
• Accession: NZ_CP034801
• Organism: Escherichia coli strain 2010C-3142

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: AYR10_RS23965
• Protein ID: WP_000813258.1
• Coordinates: 4761743..4761898 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	4761673..4761731 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYR10_RS23925	4757875..4758501	+	627	WP_127821357.1	prepilin peptidase	-
AYR10_RS23930	4758494..4758955	+	462	WP_000139447.1	replication protein	-
AYR10_RS23935	4758989..4759705	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
AYR10_RS23940	4759738..4760019	+	282	WP_000603384.1	DNA-binding protein	-
AYR10_RS23945	4760016..4760243	+	228	WP_000699809.1	hypothetical protein	-
AYR10_RS23950	4760236..4760547	+	312	WP_001289673.1	hypothetical protein	-
AYR10_RS23955	4760675..4760893	+	219	WP_000683609.1	DUF4014 family protein	-
AYR10_RS23960	4760895..4761452	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	4761673..4761731	-	59	-	-	Antitoxin
AYR10_RS23965	4761743..4761898	+	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
AYR10_RS23970	4762018..4762362	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
AYR10_RS23975	4762484..4762756	+	273	WP_000191872.1	hypothetical protein	-
AYR10_RS23980	4762758..4763807	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
AYR10_RS23985	4763820..4764125	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
AYR10_RS23990	4764188..4764742	+	555	WP_000640035.1	DUF1133 family protein	-
AYR10_RS23995	4764967..4765164	+	198	WP_000917763.1	hypothetical protein	-
AYR10_RS24000	4765300..4766013	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
AYR10_RS24015	4766464..4766895	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	4698564..4799251	100687

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T116748 WP_000813258.1 NZ_CP034801:4761743-4761898 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T116748 NZ_CP034801:4761743-4761898 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT116748 NZ_CP034801:c4761731-4761673 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin