Detailed information of TA system TA116724

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 1050182..1050721
• Replicon: chromosome
• Accession: NZ_CP034801
• Organism: Escherichia coli strain 2010C-3142

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: AYR10_RS05130
• Protein ID: WP_001303998.1
• Coordinates: 1050461..1050721 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: C3TP07
• Locus tag: AYR10_RS05125
• Protein ID: WP_000729705.1
• Coordinates: 1050182..1050442 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYR10_RS05105	1045936..1046721	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
AYR10_RS05110	1046693..1048405	+	1713	Protein_961	flagellar biosynthesis protein FlhA	-
AYR10_RS05115	1048550..1049047	-	498	Protein_962	REP-associated tyrosine transposase RayT	-
AYR10_RS05120	1049223..1049996	-	774	WP_000543891.1	C40 family peptidase	-
AYR10_RS05125	1050182..1050442	+	261	WP_000729705.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
AYR10_RS05130	1050461..1050721	+	261	WP_001303998.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
AYR10_RS05135	1050877..1051617	+	741	WP_001225679.1	murein L,D-transpeptidase	-
AYR10_RS05140	1051588..1052355	-	768	WP_001301698.1	class II glutamine amidotransferase	-
AYR10_RS05145	1052460..1053038	-	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-85)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10026.71 Da
• Isoelectric Point: 10.3188

>T116724 WP_001303998.1 NZ_CP034801:1050461-1050721 [Escherichia coli]
MNSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGT
HAALFG

Nucleotide

• Length: 261 bp

>T116724 NZ_CP034801:1050461-1050721 [Escherichia coli]
TTGAACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAATAAATTGAAATATCTTAT
GACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAGGTTCATGGAAAGGTTATC
GCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGATTTGAGAGAACTGGAACT
CACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9377.74 Da
• Isoelectric Point: 5.0758

>AT116724 WP_000729705.1 NZ_CP034801:1050182-1050442 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Nucleotide

• Length: 261 bp

>AT116724 NZ_CP034801:1050182-1050442 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA