Detailed information of TA system TA116723

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1044064..1044758
• Replicon: chromosome
• Accession: NZ_CP034801
• Organism: Escherichia coli strain 2010C-3142

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: AYR10_RS05090
• Protein ID: WP_001263495.1
• Coordinates: 1044064..1044462 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: AYR10_RS05095
• Protein ID: WP_000554757.1
• Coordinates: 1044465..1044758 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	1039651..1039731	-	81	NuclAT_11	-	-
-	1039651..1039731	-	81	NuclAT_11	-	-
-	1039651..1039731	-	81	NuclAT_11	-	-
-	1039651..1039731	-	81	NuclAT_11	-	-
AYR10_RS05065	1040327..1040785	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
AYR10_RS05070	1041046..1042503	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
AYR10_RS05075	1042560..1043081	-	522	Protein_954	peptide chain release factor H	-
AYR10_RS05080	1043080..1043283	-	204	Protein_955	RNA ligase RtcB family protein	-
AYR10_RS05085	1043602..1044054	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
AYR10_RS05090	1044064..1044462	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
AYR10_RS05095	1044465..1044758	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
AYR10_RS05100	1044810..1045865	-	1056	WP_001226186.1	DNA polymerase IV	-
AYR10_RS05105	1045936..1046721	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
AYR10_RS05110	1046693..1048405	+	1713	Protein_961	flagellar biosynthesis protein FlhA	-
AYR10_RS05115	1048550..1049047	-	498	Protein_962	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T116723 WP_001263495.1 NZ_CP034801:c1044462-1044064 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T116723 NZ_CP034801:c1044462-1044064 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT116723 WP_000554757.1 NZ_CP034801:c1044758-1044465 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT116723 NZ_CP034801:c1044758-1044465 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6