Detailed information of TA system TA116688

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1050862..1051556
• Replicon: chromosome
• Accession: NZ_CP034799
• Organism: Escherichia coli strain 2009C-4687

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: A0I37_RS05150
• Protein ID: WP_001263495.1
• Coordinates: 1050862..1051260 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: A0I37_RS05155
• Protein ID: WP_000554757.1
• Coordinates: 1051263..1051556 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	1046449..1046529	-	81	NuclAT_11	-	-
-	1046449..1046529	-	81	NuclAT_11	-	-
-	1046449..1046529	-	81	NuclAT_11	-	-
-	1046449..1046529	-	81	NuclAT_11	-	-
A0I37_RS05125	1047125..1047583	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
A0I37_RS05130	1047844..1049301	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
A0I37_RS05135	1049358..1049879	-	522	Protein_962	peptide chain release factor H	-
A0I37_RS05140	1049878..1050081	-	204	Protein_963	RNA ligase RtcB family protein	-
A0I37_RS05145	1050400..1050852	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
A0I37_RS05150	1050862..1051260	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
A0I37_RS05155	1051263..1051556	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
A0I37_RS05160	1051608..1052663	-	1056	WP_001226186.1	DNA polymerase IV	-
A0I37_RS05165	1052734..1053519	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
A0I37_RS05170	1053491..1055203	+	1713	Protein_969	flagellar biosynthesis protein FlhA	-
A0I37_RS05175	1055348..1055845	-	498	Protein_970	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1012703..1056794	44091

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T116688 WP_001263495.1 NZ_CP034799:c1051260-1050862 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T116688 NZ_CP034799:c1051260-1050862 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT116688 WP_000554757.1 NZ_CP034799:c1051556-1051263 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT116688 NZ_CP034799:c1051556-1051263 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6