Detailed information of TA system TA116673

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 4016973..4017667
• Replicon: chromosome
• Accession: NZ_CP034797
• Organism: Escherichia coli strain 08-3918

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: AYP37_RS20745
• Protein ID: WP_001263495.1
• Coordinates: 4017269..4017667 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: AYP37_RS20740
• Protein ID: WP_000554757.1
• Coordinates: 4016973..4017266 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYP37_RS20720	4012684..4013181	+	498	Protein_3837	REP-associated tyrosine transposase RayT	-
AYP37_RS20725	4013326..4015038	-	1713	Protein_3838	flagellar biosynthesis protein FlhA	-
AYP37_RS20730	4015010..4015795	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
AYP37_RS20735	4015866..4016921	+	1056	WP_001226186.1	DNA polymerase IV	-
AYP37_RS20740	4016973..4017266	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
AYP37_RS20745	4017269..4017667	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
AYP37_RS20750	4017677..4018129	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
AYP37_RS20755	4018448..4018651	+	204	Protein_3844	RNA ligase RtcB family protein	-
AYP37_RS20760	4018650..4019171	+	522	Protein_3845	peptide chain release factor H	-
AYP37_RS20765	4019228..4020685	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
AYP37_RS20770	4020946..4021404	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	4022000..4022080	+	81	NuclAT_11	-	-
-	4022000..4022080	+	81	NuclAT_11	-	-
-	4022000..4022080	+	81	NuclAT_11	-	-
-	4022000..4022080	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T116673 WP_001263495.1 NZ_CP034797:4017269-4017667 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T116673 NZ_CP034797:4017269-4017667 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT116673 WP_000554757.1 NZ_CP034797:4016973-4017266 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT116673 NZ_CP034797:4016973-4017266 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6