Detailed information of TA system TA116672

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 4011010..4011549
• Replicon: chromosome
• Accession: NZ_CP034797
• Organism: Escherichia coli strain 08-3918

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: AYP37_RS20705
• Protein ID: WP_001303998.1
• Coordinates: 4011010..4011270 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: C3TP07
• Locus tag: AYP37_RS20710
• Protein ID: WP_000729705.1
• Coordinates: 4011289..4011549 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
AYP37_RS20690	4008693..4009271	+	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-
AYP37_RS20695	4009376..4010143	+	768	WP_001301698.1	class II glutamine amidotransferase	-
AYP37_RS20700	4010114..4010854	-	741	WP_001225679.1	murein L,D-transpeptidase	-
AYP37_RS20705	4011010..4011270	-	261	WP_001303998.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
AYP37_RS20710	4011289..4011549	-	261	WP_000729705.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
AYP37_RS20715	4011735..4012508	+	774	WP_000543891.1	C40 family peptidase	-
AYP37_RS20720	4012684..4013181	+	498	Protein_3837	REP-associated tyrosine transposase RayT	-
AYP37_RS20725	4013326..4015038	-	1713	Protein_3838	flagellar biosynthesis protein FlhA	-
AYP37_RS20730	4015010..4015795	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-85)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10026.71 Da
• Isoelectric Point: 10.3188

>T116672 WP_001303998.1 NZ_CP034797:c4011270-4011010 [Escherichia coli]
MNSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGT
HAALFG

Nucleotide

• Length: 261 bp

>T116672 NZ_CP034797:c4011270-4011010 [Escherichia coli]
TTGAACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAATAAATTGAAATATCTTAT
GACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAGGTTCATGGAAAGGTTATC
GCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGATTTGAGAGAACTGGAACT
CACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9377.74 Da
• Isoelectric Point: 5.0758

>AT116672 WP_000729705.1 NZ_CP034797:c4011549-4011289 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Nucleotide

• Length: 261 bp

>AT116672 NZ_CP034797:c4011549-4011289 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA