Detailed information of TA system TA116605

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 96023..96717
• Replicon: chromosome
• Accession: NZ_CP034794
• Organism: Escherichia coli strain 06-3462

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: A1D74_RS00470
• Protein ID: WP_001263495.1
• Coordinates: 96319..96717 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: A1D74_RS00465
• Protein ID: WP_000554757.1
• Coordinates: 96023..96316 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A1D74_RS00445	91734..92231	+	498	Protein_79	REP-associated tyrosine transposase RayT	-
A1D74_RS00450	92376..94088	-	1713	Protein_80	flagellar biosynthesis protein FlhA	-
A1D74_RS00455	94060..94845	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
A1D74_RS00460	94916..95971	+	1056	WP_001226186.1	DNA polymerase IV	-
A1D74_RS00465	96023..96316	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
A1D74_RS00470	96319..96717	+	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
A1D74_RS00475	96727..97179	+	453	WP_001059871.1	GNAT family N-acetyltransferase	-
A1D74_RS00480	97498..97701	+	204	Protein_86	RNA ligase RtcB family protein	-
A1D74_RS00485	97700..98221	+	522	Protein_87	peptide chain release factor H	-
A1D74_RS00490	98278..99735	-	1458	WP_001293020.1	cytosol nonspecific dipeptidase	-
A1D74_RS00495	99996..100454	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	101050..101130	+	81	NuclAT_11	-	-
-	101050..101130	+	81	NuclAT_11	-	-
-	101050..101130	+	81	NuclAT_11	-	-
-	101050..101130	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T116605 WP_001263495.1 NZ_CP034794:96319-96717 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T116605 NZ_CP034794:96319-96717 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT116605 WP_000554757.1 NZ_CP034794:96023-96316 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT116605 NZ_CP034794:96023-96316 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6