Detailed information of TA system TA116604

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): DinJ-YafQ (relBE)/YafQ-RelB
• Location: 90060..90599
• Replicon: chromosome
• Accession: NZ_CP034794
• Organism: Escherichia coli strain 06-3462

Toxin (Protein)

• Gene name: yafQ
• Uniprot ID: -
• Locus tag: A1D74_RS00430
• Protein ID: WP_001303998.1
• Coordinates: 90060..90320 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: C3TP07
• Locus tag: A1D74_RS00435
• Protein ID: WP_000729705.1
• Coordinates: 90339..90599 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A1D74_RS00415	87743..88321	+	579	WP_000284050.1	D-sedoheptulose 7-phosphate isomerase	-
A1D74_RS00420	88426..89193	+	768	WP_001301698.1	class II glutamine amidotransferase	-
A1D74_RS00425	89164..89904	-	741	WP_001225679.1	murein L,D-transpeptidase	-
A1D74_RS00430	90060..90320	-	261	WP_001303998.1	type II toxin-antitoxin system mRNA interferase toxin YafQ	Toxin
A1D74_RS00435	90339..90599	-	261	WP_000729705.1	type II toxin-antitoxin system antitoxin DinJ	Antitoxin
A1D74_RS00440	90785..91558	+	774	WP_000543891.1	C40 family peptidase	-
A1D74_RS00445	91734..92231	+	498	Protein_79	REP-associated tyrosine transposase RayT	-
A1D74_RS00450	92376..94088	-	1713	Protein_80	flagellar biosynthesis protein FlhA	-
A1D74_RS00455	94060..94845	+	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (3-85)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-85)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 10026.71 Da
• Isoelectric Point: 10.3188

>T116604 WP_001303998.1 NZ_CP034794:c90320-90060 [Escherichia coli]
MNSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGT
HAALFG

Nucleotide

• Length: 261 bp

>T116604 NZ_CP034794:c90320-90060 [Escherichia coli]
TTGAACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAATAAATTGAAATATCTTAT
GACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAGGTTCATGGAAAGGTTATC
GCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGATTTGAGAGAACTGGAACT
CACGCGGCGCTCTTTGGGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9377.74 Da
• Isoelectric Point: 5.0758

>AT116604 WP_000729705.1 NZ_CP034794:c90599-90339 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGV

Nucleotide

• Length: 261 bp

>AT116604 NZ_CP034794:c90599-90339 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAGTTTAA