Detailed information of TA system TA116590

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3496984..3497678
• Replicon: chromosome
• Accession: NZ_CP034792
• Organism: Escherichia coli strain 2009C-3378

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: C3TNX7
• Locus tag: DR788_RS18340
• Protein ID: WP_001263495.1
• Coordinates: 3496984..3497382 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: DR788_RS18345
• Protein ID: WP_000554757.1
• Coordinates: 3497385..3497678 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3492571..3492651	-	81	NuclAT_11	-	-
-	3492571..3492651	-	81	NuclAT_11	-	-
-	3492571..3492651	-	81	NuclAT_11	-	-
-	3492571..3492651	-	81	NuclAT_11	-	-
DR788_RS18315	3493247..3493705	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
DR788_RS18320	3493966..3495423	+	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
DR788_RS18325	3495480..3496001	-	522	Protein_3464	peptide chain release factor H	-
DR788_RS18330	3496000..3496203	-	204	Protein_3465	RNA ligase RtcB family protein	-
DR788_RS18335	3496522..3496974	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
DR788_RS18340	3496984..3497382	-	399	WP_001263495.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
DR788_RS18345	3497385..3497678	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
DR788_RS18350	3497730..3498785	-	1056	WP_001226186.1	DNA polymerase IV	-
DR788_RS18355	3498856..3499641	-	786	WP_000207556.1	putative lateral flagellar export/assembly protein LafU	-
DR788_RS18360	3499613..3501325	+	1713	Protein_3471	flagellar biosynthesis protein FlhA	-
DR788_RS18365	3501470..3501967	-	498	Protein_3472	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15473.86 Da
• Isoelectric Point: 8.0949

>T116590 WP_001263495.1 NZ_CP034792:c3497382-3496984 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T116590 NZ_CP034792:c3497382-3496984 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAGCTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACTTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAATCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT116590 WP_000554757.1 NZ_CP034792:c3497678-3497385 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT116590 NZ_CP034792:c3497678-3497385 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAATTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1FJN6