Detailed information of TA system TA116581

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 1731844..1732069
• Replicon: chromosome
• Accession: NZ_CP034792
• Organism: Escherichia coli strain 2009C-3378

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: A0A1Q4PF16
• Locus tag: DR788_RS09210
• Protein ID: WP_000813258.1
• Coordinates: 1731914..1732069 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	1731844..1731902 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DR788_RS09160	1726909..1727151	+	243	WP_000747948.1	hypothetical protein	-
DR788_RS09165	1727135..1727560	+	426	WP_000693878.1	Rha family transcriptional regulator	-
DR788_RS09170	1727629..1728672	+	1044	WP_001262402.1	hypothetical protein	-
DR788_RS09175	1728665..1729126	+	462	WP_000139447.1	replication protein	-
DR788_RS09180	1729160..1729876	+	717	WP_000450627.1	DUF1627 domain-containing protein	-
DR788_RS09185	1729909..1730190	+	282	WP_000603384.1	DNA-binding protein	-
DR788_RS09190	1730187..1730414	+	228	WP_000699809.1	hypothetical protein	-
DR788_RS09195	1730407..1730718	+	312	WP_001289673.1	hypothetical protein	-
DR788_RS09200	1730846..1731064	+	219	WP_000683609.1	DUF4014 family protein	-
DR788_RS09205	1731066..1731623	+	558	WP_000104474.1	DUF551 domain-containing protein	-
-	1731844..1731902	-	59	-	-	Antitoxin
DR788_RS09210	1731914..1732069	+	156	WP_000813258.1	type I toxin-antitoxin system Hok family toxin	Toxin
DR788_RS09215	1732189..1732533	+	345	WP_000756596.1	YgiW/YdeI family stress tolerance OB fold protein	-
DR788_RS09220	1732655..1732927	+	273	WP_000191872.1	hypothetical protein	-
DR788_RS09225	1732929..1733978	+	1050	WP_001265229.1	DUF968 domain-containing protein	-
DR788_RS09230	1733991..1734296	+	306	WP_001217444.1	RusA family crossover junction endodeoxyribonuclease	-
DR788_RS09235	1734359..1734913	+	555	WP_000640035.1	DUF1133 family protein	-
DR788_RS09240	1735138..1735335	+	198	WP_000917763.1	hypothetical protein	-
DR788_RS09245	1735471..1736184	+	714	WP_000301797.1	helix-turn-helix domain-containing protein	-
DR788_RS09260	1736635..1737066	+	432	WP_001302123.1	tellurite resistance protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleG7'	1671178..1769418	98240

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5767.95 Da
• Isoelectric Point: 7.7942

>T116581 WP_000813258.1 NZ_CP034792:1731914-1732069 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFVDYESEK

Nucleotide

• Length: 156 bp

>T116581 NZ_CP034792:1731914-1732069 [Escherichia coli]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTGCGAATCCGAACCGGTCAGACGGAGGTCGCTGTCTTCGTAGACTACGAATCTGAGAAGTAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT116581 NZ_CP034792:c1731902-1731844 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A1Q4PF16

Antitoxin