Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1811386..1811888 Replicon chromosome
Accession NZ_CP034787
Organism Escherichia coli strain ECCNB20-2

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CRG85_RS08700 Protein ID WP_000767829.1
Coordinates 1811634..1811888 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CRG85_RS08695 Protein ID WP_001259255.1
Coordinates 1811386..1811637 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CRG85_RS08670 1806564..1807631 - 1068 WP_032248774.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CRG85_RS08675 1807631..1808701 - 1071 WP_032248775.1 histidinol-phosphate transaminase -
CRG85_RS08680 1808698..1810002 - 1305 WP_001572810.1 histidinol dehydrogenase -
CRG85_RS08685 1810008..1810907 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
CRG85_RS08690 1811053..1811103 - 51 WP_001364200.1 his operon leader peptide -
CRG85_RS08695 1811386..1811637 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CRG85_RS08700 1811634..1811888 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CRG85_RS08705 1811971..1812795 + 825 WP_000754758.1 SDR family oxidoreductase -
CRG85_RS08710 1812841..1813770 + 930 WP_000803351.1 LysR family transcriptional regulator -
CRG85_RS08715 1813985..1814047 + 63 WP_010723108.1 membrane protein YoeI -
CRG85_RS08720 1814037..1815395 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CRG85_RS08725 1815574..1816632 + 1059 WP_000492340.1 transport protein YeeE -
CRG85_RS08730 1816646..1816873 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T116547 WP_000767829.1 NZ_CP034787:1811634-1811888 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T116547 NZ_CP034787:1811634-1811888 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTTTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT116547 WP_001259255.1 NZ_CP034787:1811386-1811637 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT116547 NZ_CP034787:1811386-1811637 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References