Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 3527548..3528089 Replicon chromosome
Accession NZ_CP034784
Organism Escherichia coli strain ECZP248

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag ENE68_RS17370 Protein ID WP_000615976.1
Coordinates 3527811..3528089 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag ENE68_RS17365 Protein ID WP_000729704.1
Coordinates 3527548..3527808 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ENE68_RS17345 (3523223) 3523223..3524008 - 786 WP_000207587.1 putative lateral flagellar export/assembly protein LafU -
ENE68_RS17350 (3523980) 3523980..3525692 + 1713 Protein_3391 flagellar biosynthesis protein FlhA -
ENE68_RS17355 (3525916) 3525916..3526413 - 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
ENE68_RS17360 (3526589) 3526589..3527362 - 774 WP_015675129.1 C40 family peptidase -
ENE68_RS17365 (3527548) 3527548..3527808 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
ENE68_RS17370 (3527811) 3527811..3528089 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
ENE68_RS17375 (3528245) 3528245..3528985 + 741 WP_001225679.1 peptidoglycan meso-diaminopimelic acid protein amidase -
ENE68_RS17380 (3528956) 3528956..3529723 - 768 WP_000333379.1 class II glutamine amidotransferase -
ENE68_RS17385 (3529929) 3529929..3530507 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T116535 WP_000615976.1 NZ_CP034784:3527811-3528089 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T116535 NZ_CP034784:3527811-3528089 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT116535 WP_000729704.1 NZ_CP034784:3527548-3527808 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT116535 NZ_CP034784:3527548-3527808 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References