Detailed information of TA system TA116534

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3521351..3522045
• Replicon: chromosome
• Accession: NZ_CP034784
• Organism: Escherichia coli strain ECZP248

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: ENE68_RS17330
• Protein ID: WP_001263493.1
• Coordinates: 3521351..3521749 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: ENE68_RS17335
• Protein ID: WP_000554757.1
• Coordinates: 3521752..3522045 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
- (3517011)	3517011..3517091	-	81	NuclAT_9	-	-
- (3517011)	3517011..3517091	-	81	NuclAT_9	-	-
- (3517011)	3517011..3517091	-	81	NuclAT_9	-	-
- (3517011)	3517011..3517091	-	81	NuclAT_9	-	-
ENE68_RS17300 (3516351)	3516351..3517595	-	1245	WP_000189541.1	esterase FrsA	-
ENE68_RS17305 (3517687)	3517687..3518145	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
ENE68_RS17310 (3518406)	3518406..3519863	+	1458	WP_089075657.1	cytosol nonspecific dipeptidase	-
ENE68_RS17315 (3519920)	3519920..3520441	-	522	Protein_3384	peptide chain release factor H	-
ENE68_RS17320 (3520440)	3520440..3520643	-	204	Protein_3385	RtcB family protein	-
ENE68_RS17325 (3520889)	3520889..3521341	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
ENE68_RS17330 (3521351)	3521351..3521749	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
ENE68_RS17335 (3521752)	3521752..3522045	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
ENE68_RS17340 (3522097)	3522097..3523152	-	1056	WP_001226164.1	DNA polymerase IV	-
ENE68_RS17345 (3523223)	3523223..3524008	-	786	WP_000207587.1	putative lateral flagellar export/assembly protein LafU	-
ENE68_RS17350 (3523980)	3523980..3525692	+	1713	Protein_3391	flagellar biosynthesis protein FlhA	-
ENE68_RS17355 (3525916)	3525916..3526413	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T116534 WP_001263493.1 NZ_CP034784:c3521749-3521351 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T116534 NZ_CP034784:c3521749-3521351 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT116534 WP_000554757.1 NZ_CP034784:c3522045-3521752 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT116534 NZ_CP034784:c3522045-3521752 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6