Detailed information of TA system TA116484

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3567520..3568177
• Replicon: chromosome
• Accession: NZ_CP034769
• Organism: Enterobacter sp. N18-03635

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: ELK40_RS17740
• Protein ID: WP_127732082.1
• Coordinates: 3567520..3567819 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1Z3MZ32
• Locus tag: ELK40_RS17745
• Protein ID: WP_063408808.1
• Coordinates: 3567830..3568177 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ELK40_RS17730	3563902..3566100	+	2199	WP_115876107.1	type I secretion system permease/ATPase	-
ELK40_RS17735	3566111..3567280	+	1170	WP_086374946.1	HlyD family efflux transporter periplasmic adaptor subunit	-
ELK40_RS17740	3567520..3567819	+	300	WP_127732082.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ELK40_RS17745	3567830..3568177	+	348	WP_063408808.1	HigA family addiction module antidote protein	Antitoxin
ELK40_RS17750	3568198..3568716	-	519	WP_115876108.1	hypothetical protein	-
ELK40_RS17755	3568802..3569983	-	1182	WP_115876109.1	PLP-dependent aminotransferase family protein	-
ELK40_RS17760	3570004..3570891	-	888	WP_115876110.1	LysR family transcriptional regulator	-
ELK40_RS17765	3570989..3571591	+	603	WP_023308908.1	short chain dehydrogenase	-
ELK40_RS17770	3571708..3573087	-	1380	WP_115876111.1	amidohydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-95)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (8-86)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11734.39 Da
• Isoelectric Point: 10.1756

>T116484 WP_127732082.1 NZ_CP034769:3567520-3567819 [Enterobacter sp. N18-03635]
MISYFRDQWLEDFFLYGRSSNVIPSNLETALARKLDIIRAATSHRDLRSPPGNMYEALNPPLKGYSSIRVNRQYRLVFRW
KEGKAEDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T116484 NZ_CP034769:3567520-3567819 [Enterobacter sp. N18-03635]
ATGATTAGTTATTTTCGGGATCAATGGCTTGAGGATTTTTTTCTTTACGGCAGATCGAGCAATGTTATCCCTTCAAATCT
GGAAACAGCGCTTGCGAGAAAGCTCGATATCATCAGGGCAGCAACCTCGCACAGGGATTTGCGATCGCCACCGGGTAATA
TGTATGAAGCATTGAATCCTCCGCTGAAGGGCTATTCCTCCATCCGGGTAAACAGGCAATACAGGCTCGTATTTCGCTGG
AAAGAGGGTAAAGCAGAAGATCTGTACCTCTCTCCACACAAGTACACGCAACACAAGTGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13039.98 Da
• Isoelectric Point: 7.0134

>AT116484 WP_063408808.1 NZ_CP034769:3567830-3568177 [Enterobacter sp. N18-03635]
MTLQQALRKPTTPGDVLQYEYLEPLNLKISDLAEMLNVHRNTISALVNNNRKLTADMAIKLAKAFDTTIEFWLNLQLNVD
IWEAQSNARTQEELSRIKTVADVMAKRKSGQPDVA

Nucleotide

• Length: 348 bp

>AT116484 NZ_CP034769:3567830-3568177 [Enterobacter sp. N18-03635]
ATGACACTTCAACAGGCACTCCGCAAACCCACCACGCCGGGCGACGTGTTGCAGTATGAGTATCTTGAACCGCTCAATCT
GAAAATCAGCGATCTGGCGGAGATGCTCAATGTTCACCGCAACACCATCAGCGCGCTGGTCAATAACAATCGCAAGCTTA
CTGCCGATATGGCGATCAAGCTGGCAAAAGCCTTTGATACCACGATTGAATTCTGGCTGAACTTACAGCTGAACGTCGAT
ATCTGGGAAGCGCAATCTAACGCCAGGACGCAGGAGGAACTAAGCCGTATAAAGACCGTTGCGGACGTCATGGCTAAGCG
AAAATCTGGCCAACCGGATGTAGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1Z3MZ32