Detailed information of TA system TA116451

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4762337..4762939
• Replicon: chromosome
• Accession: NZ_CP034754
• Organism: Enterobacter hormaechei subsp. hoffmannii strain Eh1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A155DUZ6
• Locus tag: EM861_RS22885
• Protein ID: WP_022649837.1
• Coordinates: 4762628..4762939 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EM861_RS22880
• Protein ID: WP_022649836.1
• Coordinates: 4762337..4762627 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EM861_RS22865	4759835..4760737	+	903	WP_003861960.1	formate dehydrogenase subunit beta	-
EM861_RS22870	4760734..4761369	+	636	WP_006808711.1	formate dehydrogenase cytochrome b556 subunit	-
EM861_RS22875	4761366..4762295	+	930	WP_022649835.1	formate dehydrogenase accessory protein FdhE	-
EM861_RS22880	4762337..4762627	-	291	WP_022649836.1	helix-turn-helix domain-containing protein	Antitoxin
EM861_RS22885	4762628..4762939	-	312	WP_022649837.1	hypothetical protein	Toxin
EM861_RS22890	4763088..4764029	-	942	WP_022649838.1	fatty acid biosynthesis protein FabY	-
EM861_RS22895	4764074..4764511	-	438	WP_022649839.1	D-tyrosyl-tRNA(Tyr) deacylase	-
EM861_RS22900	4764508..4765389	-	882	WP_022649840.1	virulence factor BrkB family protein	-
EM861_RS22905	4765383..4765982	-	600	WP_022649841.1	glucose-1-phosphatase	-
EM861_RS22910	4766101..4766901	-	801	WP_022649842.1	DeoR/GlpR family DNA-binding transcription regulator	-
EM861_RS22915	4766936..4767832	-	897	WP_022649843.1	sugar kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12148.13 Da
• Isoelectric Point: 9.4455

>T116451 WP_022649837.1 NZ_CP034754:c4762939-4762628 [Enterobacter hormaechei subsp. hoffmannii]
MLFIETDIFTEDVKTLLDDDEYHRFQIFLATQPDYGDLIQNTGGLRKIRWLAGGKGKRSGVRVIYFHRTRECEIRLLLIY
RKGIKDDLSAGEKAILKKMIERW

Nucleotide

• Length: 312 bp

>T116451 NZ_CP034754:c4762939-4762628 [Enterobacter hormaechei subsp. hoffmannii]
ATGCTATTCATTGAAACGGATATTTTTACAGAAGACGTCAAAACGTTACTGGATGACGACGAATATCATAGGTTCCAAAT
ATTCCTGGCAACGCAACCGGATTACGGCGATCTGATCCAGAATACGGGAGGCTTGCGAAAGATTCGCTGGCTTGCTGGCG
GTAAGGGAAAGCGTAGTGGAGTCAGAGTTATCTATTTTCACCGTACGCGTGAGTGTGAAATCAGGTTGCTGCTCATCTAT
CGCAAAGGGATCAAAGATGACCTCTCCGCGGGTGAAAAAGCCATACTGAAAAAGATGATTGAGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10699.21 Da
• Isoelectric Point: 10.5888

>AT116451 WP_022649836.1 NZ_CP034754:c4762627-4762337 [Enterobacter hormaechei subsp. hoffmannii]
MDKALFERLTQSIAQINEIAEGQREPSRTFQIDAMKIKEIRQASGLSQSKFANLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVIPALTQ

Nucleotide

• Length: 291 bp

>AT116451 NZ_CP034754:c4762627-4762337 [Enterobacter hormaechei subsp. hoffmannii]
ATGGATAAGGCGCTGTTTGAGCGATTAACCCAAAGCATTGCGCAAATCAACGAAATTGCCGAGGGCCAACGAGAGCCGTC
GCGTACGTTTCAAATTGATGCGATGAAAATAAAAGAAATTCGTCAGGCGTCCGGGTTGTCGCAGAGTAAATTCGCGAACT
TAATCTCAGTTAGCGTTGATACGTTGCGTAACTGGGAACAAGGCAGACGCTCACCTACAGGCCCCGCTAAAGCGCTATTG
CGCGCCATTGCTAACGACCCTCAACATGTCATCCCAGCCCTAACGCAGTAA

Structures

Toxin

• AlphaFold DB: A0A155DUZ6