Detailed information of TA system TA116436

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3534094..3534671
• Replicon: chromosome
• Accession: NZ_CP034753
• Organism: Agromyces sp. LHK192

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: ELQ40_RS16030
• Protein ID: WP_127794584.1
• Coordinates: 3534094..3534372 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ELQ40_RS16035
• Protein ID: WP_127795360.1
• Coordinates: 3534393..3534671 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ELQ40_RS16015	3529762..3531072	+	1311	WP_127794581.1	glycosyltransferase	-
ELQ40_RS16020	3531151..3531387	-	237	WP_127794582.1	hypothetical protein	-
ELQ40_RS16025	3531573..3533801	-	2229	WP_127794583.1	DEAD/DEAH box helicase	-
ELQ40_RS16030	3534094..3534372	+	279	WP_127794584.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ELQ40_RS16035	3534393..3534671	+	279	WP_127795360.1	HigA family addiction module antidote protein	Antitoxin
ELQ40_RS16040	3534784..3536076	+	1293	WP_127794585.1	hypothetical protein	-
ELQ40_RS16045	3536073..3536726	+	654	WP_127795361.1	response regulator transcription factor	-
ELQ40_RS16050	3536862..3537866	+	1005	WP_164863669.1	alpha/beta hydrolase	-
ELQ40_RS16055	3537982..3538470	+	489	WP_205649514.1	GNAT family N-acetyltransferase	-
ELQ40_RS16060	3538467..3539489	+	1023	WP_164863671.1	YihY/virulence factor BrkB family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-76)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10339.76 Da
• Isoelectric Point: 8.5795

>T116436 WP_127794584.1 NZ_CP034753:3534094-3534372 [Agromyces sp. LHK192]
VIASFRHKGLQALYESGSKKGVQPAHAPKLTRILGLLDVAQSPADLAIPGFRTHELKGDLTGHWSIWVNGNWRVTFRFIG
QDVELVDYQDYH

Nucleotide

• Length: 279 bp

>T116436 NZ_CP034753:3534094-3534372 [Agromyces sp. LHK192]
GTGATCGCGAGCTTTCGACACAAGGGCCTCCAGGCGTTGTACGAGTCGGGCTCGAAGAAAGGCGTACAACCTGCGCACGC
GCCGAAGCTCACACGAATCCTCGGCCTCCTCGACGTGGCGCAGTCGCCCGCCGATCTCGCGATCCCGGGATTTCGTACGC
ACGAACTCAAGGGCGACCTGACCGGTCACTGGTCGATCTGGGTGAACGGCAACTGGCGAGTGACGTTCCGATTCATCGGG
CAGGATGTCGAACTCGTCGACTATCAGGACTACCACTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 9705.19 Da
• Isoelectric Point: 6.8793

>AT116436 WP_127795360.1 NZ_CP034753:3534393-3534671 [Agromyces sp. LHK192]
MKNPPHPGEIIGDDVLGELGLSVAEAAARLGVSRVTLSRVIHGHSGVSPNLAVRLERAGVGTARAWMALQVNFDLAREIE
GRPHEVVPLVVA

Nucleotide

• Length: 279 bp

>AT116436 NZ_CP034753:3534393-3534671 [Agromyces sp. LHK192]
ATGAAGAACCCACCGCACCCTGGCGAGATCATCGGCGACGACGTGCTCGGCGAGCTCGGATTGAGCGTCGCCGAGGCGGC
GGCGCGTCTCGGCGTCTCCCGCGTCACCCTCAGCCGGGTCATCCACGGGCACTCCGGGGTGAGTCCGAACCTCGCGGTCC
GCCTCGAGCGCGCCGGTGTCGGGACCGCCCGGGCCTGGATGGCGTTGCAGGTGAACTTCGATCTCGCCCGCGAGATCGAA
GGCAGGCCCCACGAGGTGGTTCCGCTCGTCGTGGCGTGA