Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4164332..4164873 Replicon chromosome
Accession NZ_CP034738
Organism Escherichia coli strain L65

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag EM854_RS20220 Protein ID WP_000615976.1
Coordinates 4164332..4164610 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag EM854_RS20225 Protein ID WP_000729704.1
Coordinates 4164613..4164873 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EM854_RS20205 4161914..4162492 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
EM854_RS20210 4162698..4163465 + 768 WP_000333380.1 class II glutamine amidotransferase -
EM854_RS20215 4163436..4164176 - 741 WP_001225679.1 murein L,D-transpeptidase -
EM854_RS20220 4164332..4164610 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
EM854_RS20225 4164613..4164873 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
EM854_RS20230 4165059..4165832 + 774 WP_000543895.1 C40 family peptidase -
EM854_RS20235 4165889..4166245 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
EM854_RS20240 4166238..4166516 - 279 WP_000598758.1 type II toxin-antitoxin system HicA family toxin -
EM854_RS20245 4166621..4168714 - 2094 WP_001597626.1 flagellar biosynthesis protein FlhA -
EM854_RS20250 4168698..4169837 - 1140 WP_104716369.1 flagellar type III secretion system protein FlhB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - rhs/PAAR / gmhA/lpcA 4148913..4166516 17603


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T116427 WP_000615976.1 NZ_CP034738:c4164610-4164332 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T116427 NZ_CP034738:c4164610-4164332 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT116427 WP_000729704.1 NZ_CP034738:c4164873-4164613 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT116427 NZ_CP034738:c4164873-4164613 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References