Detailed information of TA system TA116424

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3337880..3338482
• Replicon: chromosome
• Accession: NZ_CP034738
• Organism: Escherichia coli strain L65

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: EM854_RS16410
• Protein ID: WP_000897305.1
• Coordinates: 3337880..3338191 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: EM854_RS16415
• Protein ID: WP_000356397.1
• Coordinates: 3338192..3338482 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EM854_RS16380	3332910..3333695	+	786	WP_038996806.1	DeoR/GlpR family DNA-binding transcription regulator	-
EM854_RS16385	3333794..3334393	+	600	WP_127528461.1	glucose-1-phosphatase	-
EM854_RS16390	3334387..3335259	+	873	WP_000920762.1	virulence factor BrkB family protein	-
EM854_RS16395	3335256..3335693	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
EM854_RS16400	3335738..3336679	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
EM854_RS16405	3336743..3337651	-	909	WP_001162704.1	alpha/beta hydrolase	-
EM854_RS16410	3337880..3338191	+	312	WP_000897305.1	hypothetical protein	Toxin
EM854_RS16415	3338192..3338482	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
EM854_RS16420	3339087..3339305	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
EM854_RS16425	3339524..3339766	+	243	WP_001086388.1	hypothetical protein	-
EM854_RS16430	3340096..3341025	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
EM854_RS16435	3341022..3341657	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
EM854_RS16440	3341654..3342556	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T116424 WP_000897305.1 NZ_CP034738:3337880-3338191 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T116424 NZ_CP034738:3337880-3338191 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT116424 WP_000356397.1 NZ_CP034738:3338192-3338482 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT116424 NZ_CP034738:3338192-3338482 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7