Detailed information of TA system TA116386

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1103820..1104442
• Replicon: chromosome
• Accession: NZ_CP034688
• Organism: Pseudomonas citronellolis strain WXP-4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A127MSF6
• Locus tag: ELR50_RS04695
• Protein ID: WP_043317089.1
• Coordinates: 1103820..1104134 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ELR50_RS04700
• Protein ID: WP_043317091.1
• Coordinates: 1104137..1104442 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ELR50_RS04680 (ELR50_04660)	1099595..1100014	+	420	WP_052267801.1	LytTR family DNA-binding domain-containing protein	-
ELR50_RS04685 (ELR50_04665)	1100320..1100745	-	426	WP_043317083.1	hypothetical protein	-
ELR50_RS04690 (ELR50_04670)	1101177..1101527	+	351	WP_043317085.1	hypothetical protein	-
ELR50_RS04695 (ELR50_04675)	1103820..1104134	+	315	WP_043317089.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ELR50_RS04700 (ELR50_04680)	1104137..1104442	+	306	WP_043317091.1	helix-turn-helix domain-containing protein	Antitoxin
ELR50_RS04705	1104629..1104901	+	273	WP_269963353.1	hypothetical protein	-
ELR50_RS04710	1104847..1105122	+	276	WP_269963354.1	hypothetical protein	-
ELR50_RS04715 (ELR50_04695)	1105318..1105806	+	489	WP_237142636.1	integrase	-
ELR50_RS04725 (ELR50_04705)	1106553..1107401	+	849	WP_269963355.1	amidohydrolase family protein	-
ELR50_RS04730 (ELR50_04710)	1107496..1108881	-	1386	WP_269963356.1	oxygen-independent coproporphyrinogen III oxidase	-
ELR50_RS04735 (ELR50_04715)	1109078..1109287	+	210	WP_009619863.1	DUF2945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1087616..1117496	29880

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (43-101)

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-72)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11744.48 Da
• Isoelectric Point: 9.4385

>T116386 WP_043317089.1 NZ_CP034688:1103820-1104134 [Pseudomonas citronellolis]
MIFIETPVFTKRILALVDDETYRKLQEDLTLHPDAGAVIEGTGGVRKIRIAANGHGKRGGARVIYYHFTSASQIAFLLVY
DKASQEDLTADQKKVLRQIVENWR

Nucleotide

• Length: 315 bp

>T116386 NZ_CP034688:1103820-1104134 [Pseudomonas citronellolis]
ATGATCTTCATCGAAACGCCAGTCTTCACGAAGCGGATTCTCGCGCTAGTAGACGATGAGACCTATCGCAAGCTTCAGGA
AGACCTAACGCTCCACCCGGACGCCGGAGCAGTCATTGAAGGCACGGGTGGCGTGCGCAAGATTCGCATTGCTGCCAATG
GCCACGGGAAAAGAGGCGGCGCCCGGGTCATCTACTACCACTTCACATCCGCCTCTCAAATTGCATTCCTGCTGGTCTAT
GACAAGGCATCGCAGGAGGACCTGACTGCCGATCAGAAAAAGGTACTTCGGCAGATCGTTGAGAACTGGAGATAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11384.92 Da
• Isoelectric Point: 8.8706

>AT116386 WP_043317091.1 NZ_CP034688:1104137-1104442 [Pseudomonas citronellolis]
MDKELFAELVESVTQMDEIVRGERQPSREFHINAVKVREIRRATGLSQAKFARKINVAVGTLRNWEQGRRDPEGPARALL
RAIDNDPVHVLAALNGGPRED

Nucleotide

• Length: 306 bp

>AT116386 NZ_CP034688:1104137-1104442 [Pseudomonas citronellolis]
ATGGACAAAGAACTCTTCGCTGAGCTGGTCGAAAGCGTGACCCAGATGGACGAGATCGTGCGCGGCGAACGTCAACCTTC
CCGCGAATTCCACATCAATGCCGTGAAGGTTCGCGAAATCCGCCGCGCTACTGGCCTGTCCCAGGCCAAATTCGCCCGGA
AAATTAATGTTGCTGTCGGCACGCTGCGCAATTGGGAGCAAGGGCGCCGCGATCCCGAGGGGCCTGCTCGCGCGCTGCTC
CGAGCCATCGATAATGATCCGGTCCATGTTCTAGCTGCCCTGAACGGCGGCCCGCGAGAAGACTAA

Structures

Toxin

• AlphaFold DB: A0A127MSF6