Detailed information of TA system TA116385

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1063982..1064595
• Replicon: chromosome
• Accession: NZ_CP034688
• Organism: Pseudomonas citronellolis strain WXP-4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A1S1GEA1
• Locus tag: ELR50_RS04495
• Protein ID: WP_043273738.1
• Coordinates: 1064299..1064595 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: ELR50_RS04490
• Protein ID: WP_024128404.1
• Coordinates: 1063982..1064290 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ELR50_RS04465 (ELR50_04450)	1059254..1060174	+	921	WP_009619989.1	bestrophin family ion channel	-
ELR50_RS04470 (ELR50_04455)	1060190..1060756	+	567	WP_269963330.1	flavin reductase family protein	-
ELR50_RS04475 (ELR50_04460)	1060905..1062284	-	1380	WP_269963331.1	ATP-binding protein	-
ELR50_RS04480 (ELR50_04465)	1062262..1062951	-	690	WP_009619984.1	response regulator transcription factor	-
ELR50_RS04485 (ELR50_04470)	1063056..1063763	+	708	WP_269963332.1	response regulator transcription factor	-
ELR50_RS04490 (ELR50_04475)	1063982..1064290	-	309	WP_024128404.1	HigA family addiction module antitoxin	Antitoxin
ELR50_RS04495 (ELR50_04480)	1064299..1064595	-	297	WP_043273738.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ELR50_RS04500 (ELR50_04485)	1064818..1066221	+	1404	WP_043317061.1	efflux transporter outer membrane subunit	-
ELR50_RS04505 (ELR50_04490)	1066218..1067384	+	1167	WP_269963333.1	efflux RND transporter periplasmic adaptor subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (23-70)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11820.29 Da
• Isoelectric Point: 8.5982

>T116385 WP_043273738.1 NZ_CP034688:c1064595-1064299 [Pseudomonas citronellolis]
MIGSFRDAWLEAFFVRDRMPRQIPADIQERLFRKLQMLDDATTDADLRSPPSNHFERLQGRLRAYHSIRVNRQWRLVFHW
DGEKGEASDVYLDNHSYR

Nucleotide

• Length: 297 bp

>T116385 NZ_CP034688:c1064595-1064299 [Pseudomonas citronellolis]
ATGATCGGGAGTTTCAGGGACGCGTGGCTGGAAGCGTTCTTCGTGCGGGACAGGATGCCGCGGCAGATTCCCGCGGATAT
CCAGGAGCGCCTGTTCCGCAAGCTGCAGATGCTCGACGACGCCACCACGGACGCGGACTTGCGTAGCCCACCCAGCAACC
ATTTCGAGCGCTTGCAGGGGCGCCTGCGGGCATACCACTCGATCCGGGTGAATCGCCAATGGCGCCTGGTCTTTCATTGG
GATGGCGAAAAAGGCGAAGCCTCGGACGTCTACCTCGACAACCATTCGTACCGATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11471.20 Da
• Isoelectric Point: 11.1591

>AT116385 WP_024128404.1 NZ_CP034688:c1064290-1063982 [Pseudomonas citronellolis]
VIITERKPVSVGEVLSSEFLDPLGLTQGRLAEAMGVQRKLVNELCNDRRAVTADTALMLARVFGNSAEFWLNLQRRNDLW
AALNSPERRARIERARPLNRAA

Nucleotide

• Length: 309 bp

>AT116385 NZ_CP034688:c1064290-1063982 [Pseudomonas citronellolis]
GTGATCATCACCGAACGCAAGCCAGTCAGCGTCGGCGAGGTGCTCAGCAGCGAGTTCCTCGATCCGCTAGGCCTGACCCA
AGGCCGCCTCGCCGAAGCGATGGGGGTGCAGCGCAAGCTGGTCAACGAACTGTGCAACGACCGGAGGGCAGTGACGGCCG
ATACCGCCCTGATGCTGGCCCGGGTATTCGGCAACTCTGCGGAGTTCTGGCTCAACCTGCAGCGGCGCAACGACCTGTGG
GCGGCGTTGAATTCGCCAGAGCGACGTGCCCGCATAGAGCGGGCTCGGCCATTGAATCGGGCGGCCTGA

Structures

Toxin

• AlphaFold DB: A0A1S1GEA1