Detailed information of TA system TA116358

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 612221..612836
• Replicon: chromosome
• Accession: NZ_CP034673
• Organism: Vibrio anguillarum strain J360

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: DYL72_RS18750
• Protein ID: WP_029388214.1
• Coordinates: 612221..612508 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: DYL72_RS18755
• Protein ID: WP_001232701.1
• Coordinates: 612519..612836 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DYL72_RS18705	607986..608465	+	480	WP_017045717.1	lecithin retinol acyltransferase family protein	-
DYL72_RS18715	608607..608885	-	279	WP_010320663.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
DYL72_RS18720	608882..609166	-	285	WP_017040620.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
DYL72_RS18725	609364..609869	+	506	Protein_535	lipocalin family protein	-
DYL72_RS18735	611325..611567	+	243	WP_095661368.1	DUF1289 domain-containing protein	-
DYL72_RS18740	611687..611917	-	231	WP_013868597.1	PAS factor family protein	-
DYL72_RS18750	612221..612508	+	288	WP_029388214.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
DYL72_RS18755	612519..612836	+	318	WP_001232701.1	HigA family addiction module antidote protein	Antitoxin
DYL72_RS18765	612985..613401	+	417	WP_017045395.1	VOC family protein	-
DYL72_RS18770	613414..614385	-	972	WP_019282910.1	IS110 family transposase	-
DYL72_RS18780	614718..614951	+	234	WP_116285044.1	hypothetical protein	-
DYL72_RS18785	614958..615311	-	354	Protein_544	transposase	-
DYL72_RS18790	615395..615715	+	321	WP_081245520.1	IS66 family insertion sequence hypothetical protein	-
DYL72_RS18795	615712..616065	+	354	WP_069212062.1	IS66 family insertion sequence element accessory protein TnpB	-
DYL72_RS18800	616125..617657	+	1533	WP_069212063.1	IS66-like element ISVa9 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	610176..632066	21890
	-	inside	Integron	-	-	592798..625119	32321

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11350.93 Da
• Isoelectric Point: 6.7187

>T116358 WP_029388214.1 NZ_CP034673:612221-612508 [Vibrio anguillarum]
MALEFKDKWLEQFYEDDKRHRLIPSSIESALFRKLEILDAAQAESDLRIPPGNRFEHLEGNLKGWCSIRVNKQYRLIFQW
VDGVALNTYLDPHRY

Nucleotide

• Length: 288 bp

>T116358 NZ_CP034673:612221-612508 [Vibrio anguillarum]
ATGGCATTAGAATTTAAAGATAAGTGGTTAGAGCAGTTTTACGAGGATGATAAACGACATCGTTTAATTCCAAGTAGCAT
AGAGAGTGCTCTATTTCGGAAGCTGGAAATCTTAGATGCAGCTCAAGCTGAATCAGACTTAAGAATTCCGCCGGGTAATC
GTTTTGAACATCTTGAAGGAAATCTTAAAGGTTGGTGTTCAATTCGAGTGAACAAACAGTATCGTTTGATTTTTCAGTGG
GTTGATGGTGTTGCACTAAATACTTACTTAGACCCACATAGGTATTGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11705.63 Da
• Isoelectric Point: 10.4711

>AT116358 WP_001232701.1 NZ_CP034673:612519-612836 [Vibrio anguillarum]
MRKTKRRPVSVGEMLKVEFLEPMGITSKALAEAMGVHRNTVSNLINGGVLTAPVAIKLAAALGNTPEFWLNIQHAVDLWD
TRNRYQEEAKFVKPLFVSLEQSART

Nucleotide

• Length: 318 bp

>AT116358 NZ_CP034673:612519-612836 [Vibrio anguillarum]
ATGCGTAAGACAAAACGTCGTCCAGTTAGCGTTGGGGAAATGTTAAAAGTTGAATTTCTTGAACCAATGGGCATCACATC
AAAAGCGCTCGCTGAAGCGATGGGCGTACACAGAAATACGGTGAGTAATTTGATTAATGGTGGTGTGTTAACAGCTCCGG
TAGCAATCAAGTTAGCAGCGGCATTAGGCAACACACCAGAATTTTGGCTTAACATTCAACATGCAGTTGATCTTTGGGAT
ACGAGAAATCGTTATCAAGAAGAAGCAAAGTTTGTAAAGCCGTTGTTTGTTTCGCTGGAACAAAGCGCACGTACATAA