Detailed information of TA system TA116349

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 209193..209844
• Replicon: plasmid pPvSC3
• Accession: NZ_CP034667
• Organism: Proteus vulgaris strain PvSC3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A857SB97
• Locus tag: EKQ45_RS01230
• Protein ID: WP_042847389.1
• Coordinates: 209494..209844 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A8I2DDI8
• Locus tag: EKQ45_RS01225
• Protein ID: WP_042847390.1
• Coordinates: 209193..209492 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKQ45_RS01195	205502..205711	+	210	WP_042847397.1	hypothetical protein	-
EKQ45_RS01200	205788..206102	+	315	WP_042847396.1	hypothetical protein	-
EKQ45_RS01205	206376..206594	+	219	WP_042847394.1	hypothetical protein	-
EKQ45_RS01210	206628..207152	+	525	WP_052219403.1	hypothetical protein	-
EKQ45_RS01215	207584..207985	+	402	WP_042847392.1	hypothetical protein	-
EKQ45_RS01220	208036..209052	-	1017	WP_042847391.1	hypothetical protein	-
EKQ45_RS01225	209193..209492	-	300	WP_042847390.1	helix-turn-helix domain-containing protein	Antitoxin
EKQ45_RS01230	209494..209844	-	351	WP_042847389.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EKQ45_RS01235	210029..210592	+	564	WP_042847388.1	recombinase family protein	-
EKQ45_RS01240	210723..211070	+	348	WP_042847387.1	hypothetical protein	-
EKQ45_RS01245	211349..211675	+	327	WP_042847386.1	PerC family transcriptional regulator	-
EKQ45_RS01250	211764..212153	+	390	WP_042847385.1	hypothetical protein	-
EKQ45_RS01255	212182..212886	+	705	WP_042847384.1	hypothetical protein	-
EKQ45_RS01260	212901..213440	+	540	WP_042847383.1	hypothetical protein	-
EKQ45_RS01265	213504..214094	+	591	WP_166268309.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / floR / ant(2'')-Ia / catB8 / blaOXA-10 / ant(3'')-Ia / qacE / sul1 / tet(B) / cfr	-	1..284528	284528
	-	flank	IS/Tn	-	-	210029..210592	563

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-84)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13437.50 Da
• Isoelectric Point: 5.2661

>T116349 WP_042847389.1 NZ_CP034667:c209844-209494 [Proteus vulgaris]
MWEILTRDLFDSWFEEQNEETQIEVLAVLMILREDGPNLGRPQVDTLKGSQFPNMKELRIQVGGHPIRACFAFDPIRRGI
VLCAGDKKGKDETRFYKKLIKMADAEYAAHLLEQEK

Nucleotide

• Length: 351 bp

>T116349 NZ_CP034667:c209844-209494 [Proteus vulgaris]
ATGTGGGAAATATTAACGAGAGATCTCTTTGATTCTTGGTTCGAAGAGCAAAATGAGGAAACTCAGATTGAAGTGTTGGC
AGTGTTAATGATTTTACGTGAGGATGGCCCCAATCTTGGACGCCCTCAAGTTGACACCCTGAAGGGCTCACAGTTTCCCA
ACATGAAGGAACTGCGAATTCAGGTTGGTGGTCATCCGATTAGAGCGTGTTTCGCATTTGACCCAATTCGTCGTGGCATT
GTGTTATGTGCCGGTGATAAGAAAGGAAAAGATGAAACCCGTTTCTACAAGAAATTAATCAAGATGGCCGATGCGGAATA
TGCAGCGCACTTATTAGAGCAGGAGAAATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10990.70 Da
• Isoelectric Point: 7.0171

>AT116349 WP_042847390.1 NZ_CP034667:c209492-209193 [Proteus vulgaris]
MSTLNELLAKQSPETLAKIEDRAEEIRREITLAKIREELKLSQTELAQSLGVSQPSIAKLENVDNDPKLSTLKRYIKALG
GELSIDVTLPNGKRIGLHL

Nucleotide

• Length: 300 bp

>AT116349 NZ_CP034667:c209492-209193 [Proteus vulgaris]
ATGTCCACTTTAAATGAACTACTTGCAAAACAATCCCCCGAAACGCTGGCAAAAATTGAAGACCGAGCAGAAGAAATCCG
GCGTGAAATCACACTAGCTAAAATTCGTGAGGAACTGAAACTTTCTCAAACTGAGCTAGCTCAATCACTGGGCGTTTCGC
AACCTTCTATTGCTAAGCTTGAAAATGTTGATAACGATCCGAAGCTTTCAACTCTGAAGCGCTACATTAAAGCTTTAGGC
GGTGAACTTTCTATCGATGTAACGTTACCGAATGGTAAGAGAATTGGATTGCATCTTTGA

Structures

Toxin

• AlphaFold DB: A0A857SB97